The flow induced vibration in the nuclear fuel assembly causes the fretting wear between the fuel cladding tubes and the supporting grids. The reduction in tube thickness due to the fretting wear could be related to the serious damage on nuclear fuel assembly. In this paper, the effect of the water flow on fretting wear of nuclear fuel cladding tube against supporting grid was investigated through the fretting wear tester with water spout equipment. The test results were compared with the data conducted in the stationary water. At stationary water environment the wear debris was trapped between fretting surfaces, and then the fretting wear occurred by three-body abrasion. However, in the case of water flow, the two-body abrasive wear was the dominant wear mechanism, because the wear debris was easily removed by water flow.