The purpose of this study was to investigate the effects of Polygonati Rhizoma on the diet-induced hyperlipidemia in rats. Rats were divided into 4 groups, normal group(supplied enough water and feeds only), high fat diet administered group(supplied high fat diet for 4 weeks, Control group) and Astragali Radix administered group(supplied high fat diet and Astragali Radix lyophilization extract for 4 weeks, 397.3 mg/kg(rat) in sample A, 662.5mg/kg(rat) in sample B). Body weight, liver weight and serum lipid levels were evaluated. The water extract of Polygonati Rhizoma decreased body weight, liver weight, total cholesterol, LDL-cholesterol, triglyceride and phospolipid in high fat diet induced hyperlipidemia in rats, and increased HDL-cholesterol.