Effect of machine washing on structural behavior of core spun cotton/spandex interlock fabrics made with high, medium and low tightness factors has been investigated. Results are compared with those for similar fabrics knitted from 100 % cotton. Structural behavior of cotton/spandex and cotton fabric samples was observed under full relaxation and washing treatments up to 10th cycle. Based on these data, dimensional constants (U-values) were predicted under 95 % significance level. Higher course and wale densities, stitch densities, dimensional constant values, linear and area dimensional shrinkages are reported with cotton/spandex interlocks. After treatment levels, cotton/spandex showed the dimensional constants with lesser CV %. With increasing fabric tightness, decreasing of length shrinkages and area shrinkages and increasing of width shrinkages are reported in cotton/spandex interlock structures. Cotton/spandex interlocks showed longitudinally and highly deformed shapes after treatments. Fabric tightness affects significantly on structural behavior of cotton/ spandex and cotton structures during relaxation.