Meteorin in astrocytes has antiangiogenic activities via thrombospondin-1 (TSP-1), however, the regulatory mechanism has been unclear. Here we report that Meteorin upregulates TSP transcriptionally through luciferase reporter assays in astrocytes. Moreover, Meteorin activates $PKCdelta$ and ERK1/2 in astrocytes. Inhibition of $PKCdelta$ and ERK1/2 activities attenuated expression of TSP-1 by Meteorin in astrocytes. We, therefore, demonstrate that Meteorin activates $PKCdelta$ signaling and, in turn, increases TSP-1 expression in astrocytes to inhibit angiogenesis in the brain.