This study provides a description of the glottal characteristics of the word-initial low vowels /a, $ae$/ in terms of a set of acoustic parameters and discusses glottal configuration as their acoustic correlates. Furthermore, it examines the effect of prosodic boundary on the glottal properties of the vowels, seeking an account of the possible role of prosodic structure based on prosodic theory. Acoustic parameters reported to indicate glottal characteristics were obtained from the measurements made directly from the speech spectrum on recordings of Korean and English collected from 45 speakers. They consist of two separate groups of native Korean and native English speakers, each including both male and female speakers. Based on the three acoustic parameters of open quotient (OQ), first-formant bandwidth (B1), and spectral tilt (ST), comparisons were made between the speech of males and females, between the speech of native Korean and native English speakers, and between Korean and English produced by native Korean speakers. Acoustic analysis of the experimental data indicates that some or all glottal parameters play a crucial role in differentiating the speech groups, despite substantial interspeaker variations. Statistical analysis of the Korean data indicates prosodic strengthening with respect to the acoustic parameters B1 and OQ, suggesting acoustic enhancement in terms of the degree of glottal abduction and the glottal closure during a vibratory cycle.