This study was carried out to identify the differences of some macro and microanatomical features between Eunsasi poplar (Populus tomentiglandulosa T. Lee) and Konara oak (Quercus serrata Thunb.). Anatomical features such as vessel and fiber diameter, cross sectional area of ray parenchyma and endwall pit aperture diameter in ray parenchyma were compared. Differences of anatomical features between two species were found statistically significant. Earlywood vessel diameter in poplar and oak increased from pith to bark while it was found almost stable in latewood. Fiber diameter in poplar was higher than oak fiber. Ray area and the pit aperture diameter in endwall of ray parenchyma were found higher in oak than those in poplar.