Biodiesel fuel is already remarkable alternative fuel in many countries. So, many studies are performed on the environmental or economic effects as well as the characteristics of diesel engine fueled biodiesel in combustion and emission. In this study, an CRDI diesel engine used to commercial vehicle was fueled with diesel fuel and 20% biodiesel blended fuel (BDF 20%) with city mode in excess of 300 hours. Engine dynamometer testing was completed at regularly scheduled intervals to monitor the engine performance and exhaust emissions. The engine performance and exhaust emissions were sampled at 1 hour interval for analysis. To check the engine parts (valve and injector), the engine was inspected after test. It was concluded that there were no unusual deteriorations of the engine, or any unusual changes in engine power and exhaust emissions in spite of operation of 300 hours with BDF 20%.