In this study, the effect of standard phase difference in calibration of carbonyl compounds (CC) was evaluated by using their standards prepared in both gaseous and liquid phase. For this analysis, standards in both phases were prepared for 6 different CCs (formaldehyde (FA), acetaldehyde (AA), propionaldehyde (PA), butyraldehyde (BA), isovaleraldehyde (IA) and valeraldehyde (VA)) at similar concentration levels. Their gaseous standard was calibrated after derivatization with three types of DNPH cartridge, and their calibration results were compared against liquid-phase standards. Although there was a strong compatibility between 2 phases for CCs with lower molecular weights (e.g., formaldehyde and acetaldehyde), it was not the case for the heavier CCs. The results of our analysis indicate that the analytical bias of the heavier CCs can be significantly large (by more than a few tens of %). As a result, underestimation of hevier CCs can be significant, if their gaseous samples are quantified by liquid phase standard.