The research presented in this paper analytically examines the fire performance of flat plate buildings. The modeling parameters for the mechanical and thermal properties of materials are calibrated from relevant test data to minimize the uncertainties involved in analysis. The calibrated models are then adopted to perform a nonlinear finite element simulation on a flat plate building subjected to fire. The analysis examines the characteristics of slab deflection, in-plane deformation, membrane force, bending moment redistribution, and slab rotational deformation near the supporting columns. The numerical simulation enables the understanding of structural performance of flat plate under elevated temperature and, more importantly, identifies the high risk of punching failure at slab-column connections that may trigger large-scale failure in flat plate structures.