To examine the density effect and food concentration in the competitive output of two Bosmina species, the population growths of Bosmina fatalis were investigated by manipulating the density of B. longirostris and the concentration of algae. The B. fatalis density did not increase in conditions with abundant B. longirostris regardless of the food concentrations. The B. fatalis increased only at low densities of B. longirostris with high food concentrations. Based on the current results, a possible mechanism underlying the seasonal shift from B. longirostris to B. fatalis in Japanese eutrophic lakes will be explored below.