Objectives : The purpose of this study was to examine the relationship between the preparation of toothbrushing and the opinion of teachers in the practice of toothbrushing of elementary school students to provide information on the implementation of school-based toothbrushing programs. Methods : The subjects were 85 elementary school teachers. The statistical package SPSS 18.0 was used to obtain descriptive statistics and ${chi}^2$-test. Results : Oral health education program and toothbrushing preparation proved to be the most important priority in this study. The barriers to school-based toothbrushing guideance were limit of education time and shortage of space. School-based toothbrushing was related to oral health education program in the meanwhile school without school-based toothbrushing program needed the toothbrushing facilities. About 87.5% of the schools had not equipped with toothbrushing facilities. If the facilities were given, 82.5% of the school would implement the program. Conclusions : The elementary school is the place where the lifelong oral health program is given to the students, so it is very important to promote the school-based toothbtushing nationwide in the future.