Digital holographic particle velocity measurement system can be a promising optical tool for the measurements of three dimensional particle velocities. In this research, validation experiments for the digital holographic particle velocity measurement system were conducted with measuring the velocities of glass beads on a rotating disk. Uncertainty analysis was performed to identify the sources of all relevant errors and to evaluate their magnitudes. The measurement results of particle velocities obtained with digital holographic method are compared reasonably well with the known values within acceptable range of errors. Moreover, digital holographic method showed better performance compared with that of optical holographic system.