The purpose of this paper is to present the aperture cut-off filter for reduction of electromagnetic field penetration through a slot in conducting screen. The reduction characteristics of electric field penetration by the aperture cut-off filter are considered. In order to establish the concept of the aperture cut-off filter, the integral equation on the slot aperture field distribution is derived and solved by method of moments, and the reduction characteristics of penetration electric fields for the incident plane wave are calculated. The numerical results showed that the aperture cut-off filter for reduction of electric field penetration through the slot can be realized. To check the validity of the concept of an aperture cut-off filter and the theoretical analysis, the calculated electric field penetration of the metallic wall with narrow slot were compared with the experimental results.