As assessment of quantitative energy expenditure for effective exercise is becoming more important, many researches about the monitoring system for exercise status or result have been actively carried out. In this case, however, bicycle riders feel restrained and uncomfortable because the riders should wear a belt-type electrocardiograph or a watch-type accelerometer or GPS system during the assessment of bicycle exercise. In this study, therefore, an electrocardiograph based on textile electrode was developed for measuring ECG and calculating heart rate from the bicycle rider without feeling restraint, and smartphone application was also developed for monitoring the heart rate.