Research was initiated in greenhouse to investigate effects of polymer, calcium, perlite and chitosan on the growth of Kentucky bluegrass (KB). A total of 24 treatment combinations were used in the study. Treatments were made of water-swelling polymer (WSP), calcium, perlite, and chitosan in soil organic amendment (SOA). Significant differences were observed in germination rate, turfgrass coverage, turfgrass density and top growth among treatments. Germination rate, density and plant height varied with time after seeding. A proper mix of WSP is considered to be lower than 3% for turfgrass coverage and density. Regarding survival capability and top growth, however, it was good under 6%. Overall KB growth was more influenced by calcium and perlite than chitosan. Calcium and perlite were the most effective elements for early survival capacity and turfgrass density, respectively. But no effect was found by chitosan. Top growth increased with three elements, being perlite > calcium > chitosan. The chitosan was effective in early germination, but there was no effect on top growth until 3 weeks, when compared with others. A further study is needed for investigating the effect of these materials on the growth characteristics in mixtures of sand and SOA before a field application.