Objectives : The purpose of the study is to examine the effect of the indoor environmental factors of dental hygienists on physical symptoms. Methods : The subjects were 210 dental hygienists in dental hospitals and clinics in Seoul, Gyeonggi Province, Incheon and South Chungnam Province from May 1 to June 20, 2013. Results : There were significant differences between the presence or absence of windows in respiratory symptoms and eye symptoms. Room temperature, bad smell, and dry humidity had significant influences on physical and respiratory symptoms. Dermatological and ophthalmological symptoms were affected by dryness, smell and dusts. Dusts and bad smell also had influences on a variety of subjective symptoms. Conclusions : The indoor environments of dental hospitals and clinics affect the respiratory, dermatological, and ophthalmological symptoms because various hazardous materials are generated by the dental treatment. Therefore dental hygienists should have a correct understanding of bacteria, dusts and bio-aerosol in order to ensure the successful environmental control.