In this study, uncertainty analysis based on ITTC(International Towing Tank Conference) Recommended Procedures is carried out in the towing-tank experiment for motion responses and added resistance. The experiment was conducted for KVLCC2 model in head sea condition. The heave, pitch and added resistance were measured in different wave conditions, and the measurement was repeated up to maximum 15 times in each wave condition in order to observe the uncertainty of measured data. The uncertainty analysis was carried out by adopting the ISO-GUM(International Organization for Standardization, Guide to the Expression of Uncertainty in Measurements) method recommended by ITTC. This paper describes the details about the analysis method, uncertainty and the measured uncertainty for each source. The uncertainty analysis results are summarized as a tabular form. To validate the accuracy of the present measurement, the experimental results are compared with the results of numerical computation and other experiment. From the present uncertainty analysis, the main sources of uncertainty are identified, which can be very useful to improve the accuracy for added resistance experiment.