Purpose: The aim of the current study was to compare the muscle architecture of lower extremity using rehabilitative ultrasound imaging (RUSI) in young adults in Seoul and Hanoi. Methods: The study design was a comparative study of muscle cross-sectional area of lower extremity. Sixty healthy young subjects (Seoul group: 30, Hanoi group: 30) participated in this study. Real-time B-mode RUSI with a 7.5MHz linear transducer was used for measurement of cross-sectional area, pennation angle, and muscle thickness of the rectus femoris, tibialis anterior, and medial gastrocnemius muscles. Independent t-test was used for statistical analysis. Results: Significant difference in cross sectional area of rectus femoris was observed between Korean young adults and Hanoi young adults (p<0.01). Muscle thickness and pennation angle of tibialis anterior in Korean young adults were greater than in Hanoi young adults (p<0.01). In addition, the muscle thickness and pennation angle of the medial part of gastrocnemius muscle were greater in Korean young adults than in Hanoi young adults (p<0.01). In addition, in the results for gender, men had larger muscle architectures than women in both groups (p<0.01). Conclusion: This study, using RUSI, showed significant difference in muscle architectures of lower extremity in a diverse group of young adults RUSI.