Vibration profiles consist of two kinds of pattern, random and harmonic, at general engineering problems and the detailed vibration test mode of a target system is decided by the spectral condition that is exposed under operation. In moving mobility, random responses come generally from road source; whereas the harmonic responses are triggered from rotating machinery parts, such as combustion engine or drive shaft. Different spectral input may accumulate different damage in frequency domain since the accumulated fatigue damage dependent on the pattern of input spectrum in high cyclic loading condition. To evaluate the sensitivity of spectral damage according to different loading conditions, a linear elastic system is introduced to conduct a uniaxial vibration testing. Measured data, acceleration and strain, is analyzed using energy isocline function and then, the calculated fatigue damage is compared by different loading cases, random and harmonic.