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전체 선택해제
총 793건 검색
본문검색 결과만 보기
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Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT Profiler PCR Array and immunochemistry assays
Fang Yu, Roberta Rasotto, Hong Zhang, Shimin Pei, Bin Zhou, Xu Yang, Yipeng Jin, Di Zhang, Degui Lin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 13 359-367 (9 pages)
in normal mammary gland tissues; moreover, highly significant upregulation of WNT5A was observed in low malignancy CMTs. Downregulation was only detected for SFRP4 in malignant CMT samples. The subcellular location of β-catenin and cyclin D1 in 100 CMT samples was investigated via immunohistochemical analysis, and significantly increased expressions of β-catenin in cytoplasm and cyclin D1 in nuclei were revealed. Western blotting analysis revealed that the expression of β-catenin and LEF1... -
Alterations in antioxidant function and cell apoptosis in duck spleen exposed to molybdenum and/or cadmium
Mengmeng Zhang, Junrong Luo, Caiying Zhang, Huabin Cao, Bing Xia, Guoliang Hu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 9 193-200 (8 pages)
To investigate the effects of molybdenum (Mo) and/or cadmium (Cd) on antioxidant function and the apoptosis-related genes in duck spleens. Sixty healthy 11-day-old ducks were randomly divided into six groups of 10 ducks (control, low Mo group, high Mo, Cd, low Mo + Cd, and high Mo + Cd groups). All were fed a basal diet containing low or high dietary doses of Mo and/or Cd. Relative spleen weight, antioxidant indices, apoptosis-related gene mRNA expression levels, and ultrastructural changes were... -
Apoptosis in response to heat stress is positively associated with heat-shock protein 90 expression in chicken myocardial cells in vitro
Xiao-Hui Zhang, Hong Wu, Shu Tang, Qiao-Ning Li, Jiao Xu, Miao Zhang, Ya-Nan Su, Bin Yin, Qi-Ling Zhao, Nicole Kemper, Joerg Har 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 2 129-140 (12 pages)
To determine heat-shock protein (Hsp)90 expression is connected with cellular apoptotic response to heat stress and its mechanism, chicken (Gallus gallus) primary myocardial cells were treated with the Hsp90 promoter, aspirin, and its inhibitor, geldanamycin (GA), before heat stress. Cellular viability, heat-stressed apoptosis and reactive oxygen species level under different treatments were measured, and the expression of key proteins of the signaling pathway related to Hsp90 and their... -
Recurrence after exenteration for canine orbital malignant schwannoma
Seonmi Kang, Jungwhan Yang, Yesran Lee, Hyomyeong Pyo, Jaehoon Kim, Kangmoon Seo 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 17 115-118 (4 pages)
A 14-year-old Maltese dog presented with progressive exophthalmos and external deviation of the right eye. Ultrasonography revealed the presence of a retrobulbar mass and fine-needle aspiration cytology was performed, which detected a malignant mass. There was no evidence of metastasis on thoracic and abdominal radiography. Computed tomography showed no invasion into the bony orbit and no metastasis to the lung or lymph nodes. Exenteration was performed to remove the mass completely. Malignant... -
Temporal changes in mammalian target of rapamycin (mTOR) and phosphorylated-mTOR expressions in the hippocampal CA1 region of rat with vascular dementia
Jin-A Park, Choong-Hyun Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 2 11-16 (6 pages)
various biological processes in cells. In the present study, we investigated temporal changes in mTOR and phosphorylated-mTOR (p-mTOR) expressions in the rat hippocampal CA1 region following chronic cerebral hypoperfusion (CCH) induced by permanent bilateral common carotid arteries occlusion (2VO). The mTOR immunoreactivity in the pyramidal neurons and mTOR protein level in the hippocampal CA1 region were markedly decreased at 21 and 28 days after 2VO surgery. However, p-mTOR protein expression... -
First detection of West Nile virus in domestic pigeon in Korea
C-Yoon Kim, Hanseul Oh, Juha Song, Moonsuk Hur, Jae-Hwa Suh, Weon-Hwa Jheong, Jong-Taek Kim, Hong-Shik Oh, Jae-Hak Park 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 20 587-589 (3 pages)
West Nile virus (WNV) is a mosquito-borne zoonotic pathogen that has spread throughout Europe and the United States. Recently, WNV spread to East and Southeast Asia, and great efforts have been made in South Korea to prevent the spread of WNV from neighboring countries. In this study, we diagnosed the first case of WNV in pigeons (Columba livia domestica) residing in cities using a competitive enzyme-linked immunosorbent assay and confirmed it with nested reverse transcription polymerase chain... -
Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression
Tomohiro Osaki, Yuji Sunden, Akihiko Sugiyama, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Ito, Tomohiro Imagawa, Yosh 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 16 385-390 (6 pages)
as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdc/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are... -
Hematoporphyrin monomethyl ether combined with He?Ne laser irradiation-induced apoptosis in canine breast cancer cells through the mitochondrial pathway
Huatao Li, Jinjin Tong, Jun Bao, Damu Tang, Wenru Tian, Yun Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 13 235-242 (8 pages)
Hematoporphyrin monomethyl ether (HMME) combined with He-Ne laser irradiation is a novel and promising photodynamic therapy (PDT)-induced apoptosis that can be applied in vitro on canine breast cancer cells. However, the exact pathway responsible for HMME-PDT in canine breast cancer cells remains unknown. CHMm cells morphology and apoptosis were analyzed using optical microscope, terminal deoxynucleotidyl transferase dUTP nick end labeling fluorescein staining and DNA ladder assays. Apoptotic... -
Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
Cellular prion protein (PrP) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting... -
Production of α 1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology
Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 11 89-96 (8 pages)
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation....
