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전체 선택해제
총 619건 검색
본문검색 결과만 보기
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Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus
Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Yong Joo Kim, Jee Yong Park, Jong Hyeon Park, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 6 207-212 (6 pages)
One step TaqMan real-time reverse transcription polymerase chain reaction (R/T RT-PCR)using a set of primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for the internal ribosomal entry site, Leader sequence and 2B regions were used to detect FMD virus (FMDV). This assay specifically detected FMDV both in cell culture preparations and clinical samples, and was capable of distinguishing FMD from other viral diseases... -
Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies
Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)
specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs and IgY were developed to detect Listeria species and the sensitivity and specificity of the developed ELISA have been analyzed. The detection limit of ELISA using MAb 2B1 and HRP labeled IgY was... -
Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD
Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)
antimicrobial susceptibility tests, 45.5% of isolates showed antibiotic resistance to two or more antibiotics. Unlike the isolates from other countries, domestic isolates of E. coli O157:H7 was mainly resistant to ampicillin and tetracylines. In summary, the application of E. coli O157:H7 Rapid kit may be useful to detect E. coli O157:H7 due to its sensitivity and convenience. Moreover, combinational analysis of multiplex PCR together with RAPD can aid to survey the characteristics of isolates. -
Modification of pharmacokinetics of cefotaxime in uranyl nitrate-induced renal damage in black bengal goats
Biswa Priya Dutta, Shiben Chandra Debnath, Tapan Kumar Mandal and Animesh Kumar Chakraborty 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 1 1-3 (3 pages)
Pharmacokinetics of cefotaxime (50mg/kg, i.m.) were studied in both healthy and kidney damaged female black Bengal goats. Uranyl nitrate (0.75mg/kg) was administered intravenously, once daily for five consecutive days to induce kidney damage. The pharmacokinetic variables were calculated in both cases. Kidney damage caused several changes in the determined variables. The Cmax and Cmin of cefotaxime observed at 0.50 and 5 h in normal goats were 24.91 ± 1.51 and 1.22 ± 0.07 μg/ml, respectively,... -
Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry
Youn-Hwan Hwang, Jong-Hwan Lim, Byung-Kwon Park and Hyo-In Yun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 7 103-108 (6 pages)
Macrolides are frequently used in veterinary medicine as therapeutic and preventive agents for various diseases. It is difficult to determine macrolides simultaneously with conventional methods due to their similar structures. A simultaneous analysis for erythromycin, roxithromycin, tiamulin and tylosin with LC/MS has been developed. Separation was performed on C18 reversed phase column. Mobile phase was gradiently flowed with 10 mM ammonium acetate and methanol. The mass spectrometer was run in... -
Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)
virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR was also confirmed using other related canine virus and peripheral blood mononuclear cells (PBMC) and body secretes of healthy dogs. Of... -
Detection and molecular characterization of Hepatozoon canis, Babesia vogeli, Ehrlichia canis, and Anaplasma platys in dogs from Metro Manila, Philippines
Davin Edric V. Adao , Charles Michael T. Herrera , Luiza H. Galarion , Nicole R. Bolo , Rhodora S. Carlos , Enrique T. Car 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 10 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 2 79-88 (10 pages)
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Original Article : Evaluation of thermally cross-linked superparamagnetic iron oxide nanoparticles for the changes of concentration and toxicity on tissues of Sprague-Dawley rats
Jin Joo Hue , Hu Jang Lee , Sang Yong Jon , Sang Yoon Nam , Young Won Yun , Jong Soo Kim , Beom Jun Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 4호 8 245-252 (8 pages)
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Original Article : Anti-diabetic effects of benfotiamine on an animal model of type 2 diabetes mellitus
Kang Min Chung , Won Young Kang , Dong Geon Kim , Hyun Ju Hong , Young Jae Lee , Chang Hoon Han 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 1호 4 21-26 (6 pages)
