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Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Hyoung Joon Moon, Tae Yung Kim, Bong Kyun 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 14 349-352 (4 pages)
An indirect porcine epidemic diarrhea (PED)virus (PEDV)enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN)test by testing 46 samples from experimentally infected sows,73 samples from naive sows,and 1,024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain.Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells... -
Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 11 353-357 (5 pages)
A few members of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea... -
TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Seong-Hee Kim, Jun-Hun Kwon, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 10 345-351 (7 pages)
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the detection system (Rotor Gene 2000 detector) and dual-labeled fluorogenic probes. The gene specific labeled fluorogenic probe for the 3' non-translated region (3' NTR) was used to detect JEV. When the specificity of the assay using specific JEV primers was evaluated by testing three... -
Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes
Jin Sik Oh, Dae Sub Song, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 12 269-275 (7 pages)
the coronavirus group I, human coronavirus (HCoV-229E) and transmissible gastroenteritis virus (TGEV) which uses aminopeptidase N (APN), a 150 kDa protein, as their receptors. However, the receptor of the PEDV has not been identified yet. A virus overlay protein binding assay (VOPBA) was used to identify PEDV binding protein in permissive cells. The binding ability of PEDV to porcine APN (pAPN) and the effects of pAPN on infectivity of PEDV in Vero cells were also investigated. VOPBA identified... -
Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)
A one step reverse transcription PCR (RT-PCR) combined nested PCR was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR... -
PRRSV와 Staphylococcus hyicus 복합감염에 의한 돼지 귀 괴사 증후군 증례 보고
이승희, 정지열, 김성희, 김종완, 박중원, 강대영, 허지웅, 정예지, 소병재, 윤순식 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 12 143-146 (4 pages)
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Salmonella enterica subsp. enterica infections in eastern great egrets (Ardea alba modesta)
Hansol Jeong , Geewook Shin , Seungwon Yi , Eunju Kim , Haebeom Lee , Myeon Sik Yang , Chae Woong Lim , Bumseok Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 2호 13 129-131 (3 pages)
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Short Communication : Antibacterial and therapeutic effects of a combination of Coptidis rhizoma and Galla rhois extracts in piglets challenged with Campylobacter coli
Soo Mi Lee , Byung Wook Cho , Chang Yeol Yoo , Suk Kim , Song Ee Son , Hu Jang Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 4호 8 259-262 (4 pages)
