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101.

Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

B. M. Manjunatha*, J. P. Ravindra, P. S. P. Gupta, M. Devaraj, T. G. Honnappa, A. Krishnaswamy 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 9 153-156 (4 pages)

B. M. Manjunatha*, J. P. Ravindra, P. S. P. Gupta, M. Devaraj, T. G. Honnappa, A. Krishnaswamy 저
...
- 1,500원
The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO2 incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24C in the presence of cyto-b. Initially, the embryos were exposed to 10% ethylene glycol (EG) and 10%...
102.

Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress

Mi Na Lee, Sang Hun Lee, Min Young Lee, Yun Hee Kim, Jae Hong Park, Jung Min Ryu, Seung Pil Yun, Yu Jin Lee, Mi Ok Kim, Kwangsun 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 4 247-256 (10 pages)

Mi Na Lee, Sang Hun Lee, Min Young Lee, Yun Hee Kim, Jae Hong Park, Jung Min Ryu, Seung Pil Yun, Yu Jin Lee, Mi Ok Kim, Kwangsun 저
...
- 3,000원
Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5a- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure...
103.

The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)

Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 저
...
- 3,000원
An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7,...
104.

Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells

Guang-Zhen Jin, Xi-Jun Yin, Xian-Feng Yu, Su-Jin Cho, Hyo-Sang Lee, Hyo-Jong Lee, Il-Keun Kong* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 10 377-382 (6 pages)

Guang-Zhen Jin, Xi-Jun Yin, Xian-Feng Yu, Su-Jin Cho, Hyo-Sang Lee, Hyo-Jong Lee, Il-Keun Kong* 저
...
- 3,000원
Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We...
105.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 저
...
- 3,000원
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and...
106.

Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR

Khalid Munir 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 13 167-176 (10 pages)

Khalid Munir 저
...
- 3,000원
We have previously described the development of a onetube SYBR Green real-time RT-PCR assay for the detection and quantitation of infectious salmon anemia virus (ISAV) in various biological samples. The twofold aim of the present study was to verify that the optimized SYBR Green real-time RT-PCR conditions could detect ISAV isolates of different geographic origins, and to analyze the growth patterns of the selected ISAV isolates in the Chinook head salmon embryo (CHSE) -214 cells by this assay...
107.

Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus

Seong In Lim, Chang Hee Kweon, Dong Kun Yang, Dong Seob Tark, Jun Hun Kweon 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 14 251-254 (4 pages)

Seong In Lim, Chang Hee Kweon, Dong Kun Yang, Dong Seob Tark, Jun Hun Kweon 저
...
- 1,500원
Akabane, Aino and Chuzan virus are arthropod-borne (arbo)viruses mainly associated with reproductive failures in cattle. We investigated apoptosis in Vero cells (C-1586) infected with Akabane, Aino and Chuzan virus. The fragmentation of chromosomal DNA was simultaneously detected with the progress of cytopathic effect from 48 hr to 72 hr post infection, depending on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses, actinomycin D did not...
108.

Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 저
...
- 3,000원
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed...
109.

Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 6 125-130 (6 pages)

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 저
...
- 3,000원
A virus strain, showing cytopathic effect in Vero cell, was isolated from plasma of a fattening pig in Gyeonggi province, Korea in October 1999. The evaluation of physicochemical/biological properties of the isolate showed that the virus, KV1899, inoculated suckling mouse showed paralysis and died within 7 days post-inoculation, the mouse brain suspension had hemagglutinating activity with goose RBC. Pathogenicity of isolate was carried out by intracranial and intraperitoneal inoculation of 3-4...
110.

Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity

Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)

Sang-Hyun Kim, Yong-Hwan Kim 저
...
- 3,000원
Escherichia coli (E. coli) has ability to express thin aggregative fimbriae, known as curli, on the cell surface. Previously, a few example of curli expression in serogroup O157:H7 of enterohemorrhagic E. coli (EHEC) were reported, compared to other E. coli groups. However, significance of curliation in the EHEC pathobiology has not been described well in the literature. A highly curliated O157:H7 strain was used in this study in order to elucidate role of curliation in EHEC adherence to...

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