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Comparison of cardiac function and coronary angiography between conventional pigs and micropigs as measured by multidetector row computed tomography
Young Keun Ahn, Jung Min Ryu, Hea Chang Jeong, Yun Hyeon Kim, Myung Ho Jeong, Min Young Lee, Sang Hun Lee, Jae Hong Park, Seung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 1 121-126 (6 pages)
Pigs are the most likely source animals for cardiac xenotransplantation. However, an appropriate method for estimating the cardiac function of micropigs had not been established. Computed tomography (CT) analysis aimed at estimating cardiac function and assessing the coronary arteries has not been carried out in micropigs. This study determined the feasibility of evaluating cardiac function in a micropig model using multidetector row computed tomography (MDCT) and compared the cardiac function... -
Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE
Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 10 65-73 (9 pages)
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6... -
Comparative analysis of heart functions in micropigs and conventional pigs using echocardiography and radiography
Min Young Lee, Sang Hun Lee, Seung Gon Lee, Soo Hyun Park, Chai Yong Lee, Kye Hun Kim, Sun Ho Hwang, Sang Yub Lim, Young Keun Ah 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 2 7-14 (8 pages)
The production of miniature animals has been suggested for use in organ transplantation. At present, many of the studies about application of animal organs to human have been focused on pigs because of the number of advantages involved and due to their similarities with human. However, a physiological analysis of the organs to be transplanted has not yet been carried out. Therefore, this study analyzed whether or not there were physiological and morphological differences in the hearts of... -
Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus
Abdul Rahman Omar, Chong Lee Kim, Mohd Hair Bejo, Aini Ideris 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 6 241-247 (7 pages)
The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV challenge in specificpathogen- free (SPF) chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However, only four out of the seven chickens survived the hvIBDV challenge. Despite showing low antibody titer profiles,... -
Altered expression of thioredoxin reductase-1 in dysplastic bile ducts and cholangiocarcinoma in a hamster model
Byung-IL Yoon, Dae-Yong Kim, Ja-June Jang, Jeong-Hee Han 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 1 211-216 (6 pages)
Thioredoxin reductase 1 (TrxR) is a homodimeric selenoenzyme catalyzing thioredoxin (Trx) in an NADPHdependent manner. With regard to carcinogenesis, these redox proteins have been implicated in cell proliferation, transformation and anti-apoptosis. In the present study, using a hamster cholangiocarcinoma (ChC) model, we evaluated the immunohistochemical expression pattern of TrxR in precancerous lesions and ChCs as well as in normal bile ducts. The goal of this study was to determine the... -
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
J. P. N. Singh, Rishendra Verma, P. Chaudhuri 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 15 181-187 (7 pages)
and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied. -
Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins... -
Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea
Jae Ku Oem, Kwang Nyeon Lee, In Soo Cho, Soo Jeong Kye, Jee Yong Park, Jong Hyeon Park, Yong Joo Kim, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 5 117-124 (8 pages)
From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and... -
Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea
Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 3 197-205 (9 pages)
We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pig in Korea. In comparison with 22 fully sequenced JEV genomes currently available, we found that the 10,963- nucleotide RNA genome of KV1899 has a 13-nucelotide deletion in the 3' non-translated variable region and 53 unique nucleotide sequences including 3' non-translated region (NTR). Its single open reading frame has a total of 28...


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