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101.

Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 9 235-241 (7 pages)

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 저
...
- 3,000원
In optimized conditions, neither false specific nor nonspecific amplification occurred. The detection limits of the m-PCR method using DNA extracts from dilutions of pure cultures of bacteria were 35 pg for Y. ruckeri and F. psychrophilum and 70 pg for A. salmonicida subsp. salmonicida. It was determined that 15 CFU Y. ruckeri and F. psychrophilum and 30 CFU A. salmonicida subsp. salmonicida could be detected by m-PCR developed using genomic DNA extracted from dilutions of the suspensions. The...
102.

Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 7 221-225 (5 pages)

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 저
...
- 1,500원
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and...
103.

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun-Kyoung Lee, Byoung-Hee Lee, Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-H 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 12 165-167 (3 pages)

Hyun-Kyoung Lee, Byoung-Hee Lee, Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-H 저
...
- 1,500원
for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
104.

Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 저
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- 3,000원
with an additional washing step or the DNeasy Tissue Kit. In three experiments, when applied to detection of Salmonella Enteritidis in steamed pork, the real-time PCR coupled with single 24 h enrichment with BPW performed better than double 48 h enrichment with BPW plus RV or MKTTn. The simple real-time PCR assay using BPW proved to be a rapid and sensitive test for detection of low concentrations of Salmonella Enteritidis in steamed pork samples as compared with the conventional culture method.
105.

Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR

Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 10 67-72 (6 pages)

Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 저
...
- 3,000원
Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. equisimilis is generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable...
106.

A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 7 43-51 (9 pages)

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 저
...
- 3,000원
a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection of Salmonella spp., especially S. Typhimurium and S. Enteritidis, in beef and pork. For the specific and sensitive multiplex real-time PCR, three representative primers and probes were designed based on sequence data from...
107.

A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 6 35-42 (8 pages)

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 저
...
- 3,000원
no cross reactivity with other mycobacteria, such as M. avium complex, M. ulcerans, M. marium, M. kansasii, M. abscessus, M. asiaticum, M. phlei, M. fortuitum, M. scrofulaceum, M. intracellulare, M. smegmatis, and M. bovis. The overall assay for the detection of live MAP organisms is comparatively less expensive and quick, especially in comparison to standard MAP detection using a culture method requiring 6-8 weeks of incubation time, and is significantly less expensive than real-time PCR.
108.

Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells

Hyun Sun Cho, Seung Hee Chang, Youn Sun Chung, Ji Young Shin, Sung Jin Park, Eun Sun Lee, Soon Kyung Hwang, Jung Taek Kwon, Aras 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 4 23-28 (6 pages)

Hyun Sun Cho, Seung Hee Chang, Youn Sun Chung, Ji Young Shin, Sung Jin Park, Eun Sun Lee, Soon Kyung Hwang, Jung Taek Kwon, Aras 저
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- 3,000원
of TET on phosphoinositide 3-kinase/Akt and extracellular signal-regulated kinase (ERK) pathways since these signaling pathways are known to be responsible for cell growth and survival. TET suppressed cell proliferation and induced apoptosis in A549 human lung carcinoma cells. TET treatment resulted in a down-regulation of Akt and ERK phosphorylation in both time-/concentration- dependent manners. The inhibition of ERK using PD98059 synergistically enhanced the TET-induced apoptosis of A549 cell...
109.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens

Xin-Sheng Li, Gui-Qin Wang, Xiang-Dang Du, Bao-An Cui, Su-Mei Zhang, Jian-Zhong Shen 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 7 243-247 (5 pages)

Xin-Sheng Li, Gui-Qin Wang, Xiang-Dang Du, Bao-An Cui, Su-Mei Zhang, Jian-Zhong Shen 저
...
- 1,500원
(83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively. Molecular detection revealed that the incidence rates of the floR, cmlA, cat1, cat2 and cat3 were 29, 31, 16, 13, and 0%, respectively. Additionally, 10% of the isolates were positive for both floR and cmlA. As these antimicrobial agents may potentially induce cross-resistance between animal and human bacterial pathogens, their prudent use...
110.

Targeted surveillance to assess the presence of BSE in the age risk population of cattle slaughtered in Bursa, Turkey: preliminary results of an immunohistochemical detection study for the 2004-2005 period

M. Mufit Kahraman, M. Ozgur Ozygit, Ahmet Akkoc, Bulent Ediz, Deniz Misirlioglu, Gursel Sonmez, Aylin Alasonyalilar, Rahsan Yilm 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 15 193-195 (3 pages)

M. Mufit Kahraman, M. Ozgur Ozygit, Ahmet Akkoc, Bulent Ediz, Deniz Misirlioglu, Gursel Sonmez, Aylin Alasonyalilar, Rahsan Yilm 저
...
- 1,500원
of Turkey was assessed by the EU Scientific Steering Committee as "it is likely but not confirmed".This study presents the results of a targeted surveillance study to assess the presence of BSE in the age risk population of Bursa, Turkey. In the assessment procedure, the immunohistochemical detection of protease-resistant prion protein (PrP-Sc) was aimed at and applied to 420 brain tissues of cattle slaughtered in Bursa at an age of 30-months and older. None of the samples were positive for BSE.

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