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101.

Differential regulation of senescence and in vitro differentiation by 17β-estradiol between mesenchymal stem cells derived from male and female mini-pigs

Won-Jae Lee, Seung-Chan Lee, Jeong-Hyun Lee, Gyu-Jin Rho, Sung-Lim Lee 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 5 159-170 (12 pages)

Won-Jae Lee, Seung-Chan Lee, Jeong-Hyun Lee, Gyu-Jin Rho, Sung-Lim Lee 저
...
- 4,100원
Supplementation of 10 M E2 resulted in down-regulation of β-galactosidase activity and pro-apoptotic activity in both BMSCs, while anti-apoptotic activity was up-regulated in only Fp-BMSCs. Further, E2 increased the osteogenic ability of Fp-BMSCs. Based on these findings, optimal utilization of E2 can improve cellular senescence and apoptosis, as well as in vitro osteogenesis of BMSCs, and could therefore be useful in stem cell therapy, particularly in bone regeneration for adult females.
102.

Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 저
...
- 3,000원
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated...
103.

Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 저
...
- 3,000원
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation...
104.

In vitro development of canine somatic cell nuclear transfer embryos in different culture media 

Dong-Hoon Kim, Jin-Gu No, Mi-Kyung Choi, Dong-Hyeon Yeom, Dong-Kyo Kim, Byoung-Chul Yang, Jae Gyu Yoo, Min Kyu Kim, Hong-Tea Kim 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 14 233-235 (3 pages)

Dong-Hoon Kim, Jin-Gu No, Mi-Kyung Choi, Dong-Hyeon Yeom, Dong-Kyo Kim, Byoung-Chul Yang, Jae Gyu Yoo, Min Kyu Kim, Hong-Tea Kim 저
...
- 1,500원
of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently...
105.

The potential role of COX-2 in cancer stem cell-mediated canine mammary tumor initiation: an immunohistochemical study 

Jian Huang, Di Zhang, Fuqiang Xie, Degui Lin 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 13 225-231 (7 pages)

Jian Huang, Di Zhang, Fuqiang Xie, Degui Lin 저
...
- 3,000원
the present study were to investigate COX-2 expression patterns and their effect on CSC-mediated tumor initiation in primary canine mammary tissues and tumorsphere models using immunohistochemistry. Patterns of COX-2, CD44, octamer-binding transcription factor (Oct)-3/4, and epidermal growth factor receptor (EGFR) expression were examined in malignant mammary tumor (MMT) samples and analyzed in terms of clinicopathological characteristics. COX-2 and Oct-3/4 expression was higher in MMTs compared...
106.

Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 10 241-248 (8 pages)

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 저
...
- 3,000원
differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlândia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlândia and São Paulo). The results suggest that both antigens were able to identify animals seropositive for E.canis in Brazil since these Brazilian strains appear to be highly conserved.
107.

Development of in vitro produced porcine embryos according to serum types as macromolecule

Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)

Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 저
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- 3,000원
This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum, prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then...
108.

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

Hun-Su Seo, Kyoung Hee Kim, Dae-Yong Kim, Bong-Kyun Park, Nam-Shik Shin, Jae-Hoon Kim, Heejeong Youn 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 2 241-248 (8 pages)

Hun-Su Seo, Kyoung Hee Kim, Dae-Yong Kim, Bong-Kyun Park, Nam-Shik Shin, Jae-Hoon Kim, Heejeong Youn 저
...
- 3,000원
fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and...
109.

In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25−CD4+ cells

Tomasz Maślanka*, Jerzy Jan Jaroszewski 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 3 125-134 (10 pages)

Tomasz Maślanka*, Jerzy Jan Jaroszewski 저
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- 3,000원
The purpose of this study was to evaluate the effect of meloxicam (MEL) on selected immune parameters of bovine CD25CD4+, CD25CD4+, and CD25−CD4+ cells. Peripheral blood mononuclear cells (PBMCs) collected from 12-month-old heifers were treated with MEL at a concentration corresponding to the serum level of this medication following administration at the recommended dose (MEL 5 × 10 M) and at a concentration 10 times lower (MEL 5 × 10 M). After 12 and 24 h of incubation with the drug,...
110.

Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels

Jung-Taek Kang, Dae-Kee Kwon, Sol-Ji Park, Su-Jin Kim, Joon-Ho Moon, Ok-Jae Koo, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 3 15-20 (6 pages)

Jung-Taek Kang, Dae-Kee Kwon, Sol-Ji Park, Su-Jin Kim, Joon-Ho Moon, Ok-Jae Koo, Goo Jang, Byeong-Chun Lee* 저
...
- 3,000원
is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 ug/mL quercetin during in vitro maturation...

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