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Effects of Noradrenaline on the Spontaneous Contraction and Ionic Current in the Antral Circular Muscle of Guinea-pig Stomach
Jun, Jae-Yeoul, Lee, Sang-Jin, Kim, Sung-Joon, Suh, Jae-Yul, So, In-Suk, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 1 115-122 (8 pages)
There is evidence that noradrenaline enhances spontaneous contractions dose-dependently in guinea-pig antral circular muscle. To investigate the mechanism of this excitatory action, slow waves and membrane currents were recorded using conventional microelectrode techniques in muscle strips and the whole cell patch clamp technique in isolated gastric myocytes. On recording slow waves, noradrenaline (10-5 M) induced the hyperpolarization of the membrane potential, although the shape of the slow... -
Low-Voltage Activated Ca2+ Current Carried via T-Type Channels in the Mouse Egg
Yang, Young-Sun, Park, Young-Geun, Cho, Soo-Wan, Cheong, Seung-Jin, Haan, Jae-Hee, Park, Choon-Ok, Hong, Seong-Geun 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 10 107-114 (8 pages)
Most of voltage operated Ca2+ channels can be divided into three types (T-, N-, and L-type), according to the electrical and pharmacological properties. Their distribution is closely related to cell specific functions. Properties of the voltage activated Ca2+ current in mouse eggs were examined to classify channel types and to deduce the function by using whole cell voltage clamp technique. Ca2+ currents appeared below -40 mV and reached a maximum at -15 mV (half maximum was -31 mV), then... -
Effects of the Endothelium on the Contractile Responses to Norepinephrine in Isolated Proximal and Distal Coronary Artery of Pigs
Kim, Jong-Hoon, Jeon, Byeong-Hwa, Chang, Seok-Jong, Park, Hae-Kun 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 3 27-36 (10 pages)
Effects of the endothelium on the contractile responses to norepinephrine (NE) were investigated in isolated helical strips of the proximal and distal coronaries artery of pigs. The helical strips were immersed in Tris-buffered Tyrode s solution equilibrated with 100% O2 at 35℃ and its isometric tension was measured. NE relaxed the strips precontracted with acetylcholine from both the proximal and distal coronary artery. NE-induced relaxation, which might be induced mainly by β-adrenoceptor... -
Effect of Capsaicin on Causalgiform Pain in the Rat
Kim, Kwang-Jin, Jeon, Byeong-Hwa, Kim, Won-Sik, Park, Kyung-Ran, Choi, Sae-Jin 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 2호 6 143-150 (8 pages)
The purpose of this study is to obtain the effective concentration of capsaicin to relieve pain with no change in the number of C-fibers and its effective duration for pain relief. Capsaicin has been used extremely as a experimental tool and as topical medications for acute or chronic tissue injuries and partial nerve injury is the main cause of causalgiform pain disorders in humans. Here, the left sciatic nerve was ligated unilaterally at the high level of the thigh to prepare an animal model... -
Effect of Various Divalent Ions on the Calcium Current of Adrenal Medullary Chromaffin Cells in the Rat
Kim, Jun, Leem, Chae-Hun, Kim, Sang-Jeong 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 2호 2 113-122 (10 pages)
It is well known that chromaffin cells of adrenal medulla secrete catecholamine in response to sympathetic nerve activation and the influx of Ca2+ through the voltage dependent Ca2+ channels (VDCC) in the cell membrane do a major role in this secretory process. In this study, we explored the effect of divalent cations on VDCC of rat chromaffin cells. Rat (Sprague-Dawley rat, 150-250 gm) chromaffin cells were isolated and cultured. Standard giga seal, whole cell recording techniques were employed... -
Characteristics of Synthesized Red Cells Using Bovine Hemoglobin as Oxygen Carrier
Cho, Eng-Haeng, Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 4 37-44 (8 pages)
The encapsulation of the Purified bovine hemoglobin with Phospholipids obtained from egg folk was performed using a rotary vacuum evaporator. The prepared Hb-containing liposome (hemosome) had good properties as artificial red blood cell. The shape and size of the hemosomes were measured by a phase contrast microscope and image analyzer. The function of the hemosome as oxygen carrier was tested by measuring oxygen saturation curve with blood gas analyzer and infusing it into rats. The prepared... -
Cl--sensitive Component of Ca2+-activated Tail Current in Rabbit Atrial Myocytes
Park, Choon-Ok, So, In-Suk, Ho, Won-Kyung, Kim, Woo-Gyeum, Earm, Yung-E 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 3 27-36 (10 pages)
We used the whole cell patch clamp technique to examine the ionic basis for the tail current after depolarizing pulse in single atrial myocytes of the rabbit. We recorded the tail currents during various repolarizations after short depolarizing pulse from a holding potential of -70 mV. The potassium currents were blocked by external 4-aminopyridine and replacement of internal potassium with cesium. The current was reversed to the outward direction above +10 mV. High concentrations of... -
Ionic Dependence and Modulatory Factors of the Background Current Activated by Isoprenaline in Rabbit Ventricular Cells
Leem, Chae-Hun, Lee, Suk-Ho, So, In-Suk, Ho, Won-Kyung, Earm, Yung-E 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 2 15-26 (12 pages)
In order to elucidate the properties of the background current whole cell patch clamp studies were performed in rabbit ventricular cells. Ramp pulses of ±80 mV from holding potential of 40 mV(or 20 mV) at the speed of 0.8 V/sec were given every 30 sec(or 10 sec) and current-voltage diagrams(I-V curve) were obtained. For the activation of the background current isoprenaline, adenosine 3 ,5 -cyclic monophosphate(dBcAMP), guanosine 3 ,5 -cyclic monophosphate(cGMP), and N6-2 -o-dibutyryladenosine 3... -
The Contractile and Electrical Responses of Guinea-pig s Gastric Smooth Muscle to Serotonin
Lee, Sang-Jin, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 4 133-146 (14 pages)
In order to elucidate systematically the effects of serotonin on gastric motility of guinea-pig, the contractile and electrical responses to serotonin were recorded using four kinds of muscle strips prepared from antral circular, antral longitudinal, fundic circular, and fundic longitudinal muscles. Experiments were performed using various methods including isometric contraction recording, transmural electrical field stimulation, junction potential recording, intracellular microelectrode...


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