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1141.

Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)

Hyun-Jeong Jeong, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Bo-Sook Kim, Jung-Rae Rho, Mi-Hyun Yoo, Byung-Hoon Jeong, Yon 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 16 299-301 (3 pages)

Hyun-Jeong Jeong, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Bo-Sook Kim, Jung-Rae Rho, Mi-Hyun Yoo, Byung-Hoon Jeong, Yon 저
...
- 1,500원
Polymorphisms of the prion protein gene (PRNP) have been detected in several cervid species. In order to confirm the genetic variations, this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer (Cervus nippon laiouanus) in Korea. A total of three single-nucleotide polymorphisms (SNPs) at codons 100, 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced...
1142.

The expression and cellular localization of phospholipase Disozymes in the developing mouse testis

Seungjoon Kim, Heechul Kim, Yongduk Lee, Jin Won Hyun, Young Ho Lee, Min Kyoung Shin, Do Sik Min, Taekyun Shinn 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 1 209-212 (4 pages)

Seungjoon Kim, Heechul Kim, Yongduk Lee, Jin Won Hyun, Young Ho Lee, Min Kyoung Shin, Do Sik Min, Taekyun Shinn 저
...
- 1,500원
8 using Western blot analysis and immunohistochemistry. The expression of both PLD1 and PLD2 increased gradually with development from postnatal week 1 to 8. Immunohistochemically, PLD immunoreactivity was detected in some germ cells in the testis and interstitial Leydig cells at postnatal week 1. PLD was mainly detected in the spermatocytes and residual bodies of spermatids in the testis after 8 weeks after birth. The intense immunostaining of PLD in Leydig cells remained unchanged by postnatal...
1143.

Bilateral Polydactyly in a foal

Bianca Carstanjen, Marie Abitbol, Christophe Desbois 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 17 201-203 (3 pages)

Bianca Carstanjen, Marie Abitbol, Christophe Desbois 저
...
- 1,500원
The following case report describes the diagnosis and surgery of bilateral polydactyly of unknown origin in a colt. A 7-month-old Berber colt was referred for cosmetic and curative excision of supernumerary digits. Radiographic examination revealed bilateral polydactyly and welldeveloped first carpal bones. Surgery consisted of an osteotomy of both second metacarpal bones combined with an amputation of the supernumerary digits. The follow-up at 18 months after surgery revealed a sound horse with...
1144.

Targeted surveillance to assess the presence of BSE in the age risk population of cattle slaughtered in Bursa, Turkey: preliminary results of an immunohistochemical detection study for the 2004-2005 period

M. Mufit Kahraman, M. Ozgur Ozygit, Ahmet Akkoc, Bulent Ediz, Deniz Misirlioglu, Gursel Sonmez, Aylin Alasonyalilar, Rahsan Yilm 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 15 193-195 (3 pages)

M. Mufit Kahraman, M. Ozgur Ozygit, Ahmet Akkoc, Bulent Ediz, Deniz Misirlioglu, Gursel Sonmez, Aylin Alasonyalilar, Rahsan Yilm 저
...
- 1,500원
Bovine spongiform encephalopathy (BSE), a member of the transmissible spongiform encepahlopathies, has been a notifiable disease in Turkey since 1997. In 2002, the BSE status of Turkey was assessed by the EU Scientific Steering Committee as "it is likely but not confirmed".This study presents the results of a targeted surveillance study to assess the presence of BSE in the age risk population of Bursa, Turkey. In the assessment procedure, the immunohistochemical detection of protease-resistant...
1145.

Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

Amr E. El Hakim, Yasser E. Shahein, Amira M. K. Abouelella, Mohamed E. Selim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 12 175-180 (6 pages)

Amr E. El Hakim, Yasser E. Shahein, Amira M. K. Abouelella, Mohamed E. Selim 저
...
- 3,000원
rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.
1146.

Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro

Mohammad Alamgir Hossain, Osamu Yamato, Gonhyung Kim, Masahiro Yamasaki, Yoshimitsu Maede 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 11 169-174 (6 pages)

Mohammad Alamgir Hossain, Osamu Yamato, Gonhyung Kim, Masahiro Yamasaki, Yoshimitsu Maede 저
...
- 3,000원
The present study evaluated the effects of infected culture supernatant of erythrocytes, fractionation of culture supernatant and serum from dogs infected with Babesia gibsoni (B. gibsoni) on the maturation of canine reticulocytes in vitro. The SDS-PAGE demonstrated that significantly broader bands were generated by both the infected culture supernatant of erythrocytes and the serum from dogs chronically infected with B. gibsoni. The culture supernatant of erythrocytes infected with B. gibsoni...
1147.

PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases

Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 8 151-154 (4 pages)

Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 저
...
- 1,500원
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc),...
1148.

Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 저
...
- 3,000원
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal...
1149.

Immunohistochemical study of osteopontin in boar testis

Seungjoon Kim, Taekyun Shin 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 1 107-110 (4 pages)

Seungjoon Kim, Taekyun Shin 저
...
- 1,500원
in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets, the 66-kDa OPN band was detected in the testes, but not the 32-kDa band. In the newborn testis, OPN immunostaining was seen in gonocytes and in some supporting cells in the seminiferous tubules, as well as in interstitial Leydig cells. In the adult boar testis, OPN immunoreactivity was detected in seminiferous tubules with varying intensities....
1150.

Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 저
...
- 3,000원
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to...

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