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Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins... -
Mitral valve prolapse in Cavalier King Charles Spaniel:A review and case study
Changbaig Hyun 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 10 67-73 (7 pages)
A 5 year-old spayed female Cavalier King Charles Spaniel was presented after a 3- to 5-day onset of severe respiratory distress. The dog also had a history of several episodes of syncope prior to presentation. A comprehensive diagnostic investigation revealed a midsystolic click sound on cardiac auscultation, signs of left sided cardiac enlargement in ECG and thoracic radiography, mitral valvular leaflet protrusion into left the atrium, decreased E-point-to septal separation (EPSS) and mitral... -
Transcranial Doppler ultrasound analysis of resistive index in rostral and caudal cerebral arteries in dogs
Minho Seo, Hojung Choi, Kichang Lee, Mincheol Choi, Junghee Yoon 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 9 61-66 (6 pages)
Transcranial Doppler (TCD) was carried out to determine the resistive index (RI) values of normal canine cerebral arteries and its reproducibility and to evaluate the change of cerebral vascular resistance following diuretics administration. RI values of rostral cerebral artery (RCA) were compared between fontanelle window and temporal window. Normal ranges and reproducibility of the RI values were examined in the rostal cerebral artery (RCA) and caudal cerebral artery (CCA). And after... -
Growth performance and nutrient utilization in black Bengal bucks (Capra hircus) supplemented with graded doses of chromium as chromium chloride hexahydrate
Tarun K. Paul, Sudipto Haldar, Tapan K. Ghosh 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 5 33-40 (8 pages)
Black Bengal bucks (Capra hircus) were supplemented with 0, 0.2 or 0.4 mg elemental chromium (Cr) as chromium chloride hexahydrate per day for 70 days. Intake of dry matter (p <0.001), crude protein (p < 0.001) and neutral detergent fiber (p <0.01) increased due to Cr supplementation. The apparent total tract digestibility of dry matter (p <0.01), organic matter (p <0.05), crude protein (p <0.001) and acid detergent fiber (p <0.01) improved and the total body weight gain and the live weight gain... -
General pharmacological profiles of bee venom and its water soluble fractions in rodent models
Hyun-Woo Kim, Young-Bae Kwon, Tae-Won Ham, Dae-Hyun Roh, Seo-Yeon Yoon, Seuk-Yun Kang, Il-Suk Yang, Ho-Jae Han, Hye-Jung Lee, Al 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 5 309-318 (10 pages)
Recently, the antinociceptive and anti-inflammatory efficacy of bee venom (BV, Apis mellifera) has been confirmed in rodent models of inflammation and arthritis. Interestingly, the antinociceptive and anti-inflammatory effect of whole BV can be reproduced by two water-soluble fractions of BV (>20 kDa:BVAF1 and<10 kDa: BVAF3). Based on these scientific findings, BV and its effective water-soluble fractions have been proposed as potential anti-inflammatory and antinociceptive pharmaceuticals.... -
Ultrasonographic measurement of optic nerve sheath diameter in normal dogs
Hee-Chun Lee, Ho-Jung Choi, Min-Cheol Choi, Jung-Hee Yoon 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 11 265-268 (4 pages)
This study was carried out to assess the feasibility of ultrasonographic measurements of the optic nerve sheath diameter (ONSD) in normal dogs and evaluate the effect of breed, sex, body weight and age on biometry of ONSD. The ONSDs were evaluated in 15 dogs (10-50 months old) with normal eye (7 Yorkshire terrier and 8 Maltese). Ultrasonographic measurements of the ONSD were carried out at a constant position located 5 mm behind the optic disc. Eyes were collected immediately after euthanasia,... -
Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae
Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 4 225-228 (4 pages)
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by PCR with primer designed based on the N- and C-terminal of the toxin was cloned in TA cloning vector and sequenced. The nucleotide sequences of apxIA gene was reported to GeneBank with the accession numbers of AF363361. Identity of the Apx IA from the cloned gene in E. coli was... -
Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach
Son-il Pak, Doo Kim, Mo Salman 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 8 51-56 (6 pages)
To draw inferences about the sensitivity and specificity of the newly developed ELISA test for bovine paratuberculosis (PTB) diagnosis and posterior distribution on the prevalence of PTB in a province of Korea, we applied Bayesian approach with Gibbs sampler to the data extracted from the prevalence study in 1999. The data were from a single test results without a designated gold test. The prevalence estimates for PTB in study population ranged 3.2-5.3% for conservative and 6.7-7.1% for liberal,... -
Efficacy of Purified Vi Polysaccharide Typhoid Vaccine
Jong-Hwan Park, Jung-Joo Hong, Eun-Sil Choi, Jin-Won Leeand Jae-Hak Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 2 67-70 (4 pages)
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microg / 0.5 ml / mouse. The ELISA titer (0.50970.0606) was 4 times in the group treated with high dose (0.25 microg/0.5ml) as in control (0.11130.0110). Six... -
Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice
Hyo-ik Ryu, Chul-joong Kim 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 5 87-95 (9 pages)
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were...


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