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Development of an Improved Loop-Mediated Isothermal Amplification Assay for On-Site Diagnosis of Fire Blight in Apple and Pear
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 5 191-198 (8 pages)
Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as... -
Computer-Aided Drug Discovery in Plant Pathology
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 6호 1 529-542 (14 pages)
Control of plant diseases is largely dependent on use of agrochemicals. However, there are widening gaps between our knowledge on plant diseases gained from genetic/mechanistic studies and rapid translation of the knowledge into target-oriented development of effective agrochemicals. Here we propose that the time is ripe for computer-aided drug discovery/design (CADD) in molecular plant pathology. CADD has played a pivotal role in development of medically important molecules over the last three... -
Effect of X-irradiation on Citrus Canker Pathogen Xanthomonas citri subsp. citri of Satsuma Mandarin Fruits
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 4호 3 343-349 (7 pages)
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most important bacterial diseases of citrus. Because citrus canker is not found in many countries including European Union and Australia, Xcc is strictly regulated in order to prevent its spread. In this study, the effects of X-irradiation on Xcc growth either in the suspension or on the surface of citrus fruits were investigated. The suspension containing 1×10 cfu/ml of Xcc was irradiated with different absorbed doses... -
Roles of Forkhead-box Transcription Factors in Controlling Development, Pathogenicity, and Stress Response in Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 4 136-150 (15 pages)
Although multiple transcription factors (TFs) have been characterized via mutagenesis to understand their roles in controlling pathogenicity and infection-related development in Magnaporthe oryzae, the causal agent of rice blast, if and how forkhead-box (FOX) TFs contribute to these processes remain to be characterized. Four putative FOX TF genes were identified in the genome of M. oryzae, and phylogenetic analysis suggested that two of them (MoFKH1 and MoHCM1) correspond to Ascomycota-specific... -
Biocontrol of Late Blight (Phytophthora capsici) Disease and Growth Promotion of Pepper by Burkholderia cepacia MPC-7
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 8 67-76 (10 pages)
A chitinolytic bacterial strain having strong antifungal activity was isolated and identified as Burkholderia cepacia MPC-7 based on 16S rRNA gene analysis. MPC- 7 solubilized insoluble phosphorous in hydroxyapatite agar media. It produced gluconic acid and 2-ketogluconic acid related to the decrease in pH of broth culture. The antagonist produced benzoic acid (BA) and phenylacetic acid (PA). The authentic compounds, BA and PA, showed a broad spectrum of antimicrobial activity against yeast,... -
Development of Diagnostic Technology of Xylella fastidiosa Using Loop-Mediated Isothermal Amplification and PCR Methods
Suyoung Kim, Yujin Park, Gidon Kim 한국식물병리학회 식물병연구 7 Pages
한국식물병리학회 식물병연구 2021, 27권 1호 6 38-44 (7 pages)
Xylella fastidiosa is the most damaging pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for speci- ficity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four... -
Efficacy of Forest-Thermal Combined Therapy for Anxiety and Stress among Smoking-Cessation Attempters
Chae Youngran, Lee Sunhee, Kim So-yeon, Choi Jungkee 한국기초간호학회 Journal of Korean Biological Nursing Science 8 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2022, Volume 24, No 4 3 227-234 (8 pages)
Purpose: Smoking is a way of coping with anxiety and stress. This study aimed to identify the effects of forest-thermal combined therapy on anxiety and depression in smokers who desire to quit smoking. Methods: Thirty participants were included in the study, 15 in the experimental group and 15 in the control group. Those in the experimental group participated in a three-day forest-thermal combined therapy program. The program includes forest walks, meditation and thermal therapy in the charcoal... -
중·고등학교 보건교사의 자궁경부암 예방백신에 대한 지식과 암 정보추구행위 및 교육의지
김창희, 송주은 한국기초간호학회 Journal of Korean Biological Nursing Science 10 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2012, Vol.14 No.4 2 239-248 (10 pages)
자궁경부암은 전 세계적으로 매년 약 50만 명의 환자가 발생하고 사망률이 50%에 이르는 주요생식기 암이다(Parkin, Bary, Ferlay, & Pisani, 2005). 우리나라의 경우, 국가암관리사업의 일환으로 1999년부터 자궁경부암 검진사업이 실시된 이후 조기진단이 증가하고 자궁경부암 발생이 다소 줄었다고는 하나, 2004년 한 해 동안 3,148명의 자궁경부암 환자가 발생하였고(Han et al., 2007), 2009년 3,733명이 발생하여 여전히 여성암 중 4.0% (7위)를 차지하는 주요 질환이다. 또한 전체적인 자궁경부암의 감소추세와는 달리 35세 미만...


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