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Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine
Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)
The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR).... -
The effect of conglutinin on production of reactive oxygen species in bovine granulocytes
Marta Dec*, Andrzej Wernicki, Andrzej Puchalski, Renata Urban-Chmiel, Adam Wa?ko 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 5 33-38 (6 pages)
of collectins that bind sugar residues in a Ca-dependent manner and are effector molecules in innate immunity. Conglutinin appears to play an important role in immune defense mechanisms, showing antiviral and antibacterial activities when tested in vivo and in vitro. The present study evaluated the effect of conglutinin on the respiratory bursts in bovine peripheral phagocytes. Using nitroblue tetrazolium and hydrogen peroxide assays, we showed that sugar ligand- bound conglutinin stimulated the... -
Virulence factors in Escherichiacoli isolated from calves with diarrhea in Vietnam
Tan Duc Nguyen, Thin Thanh Vo, Hung Vu-Khac 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 8 159-164 (6 pages)
This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin... -
In vivo metabolism of Talosin A, new isoflavonol glycoside from Kitasatospora kifunensis, in rats
Jong-Hwan Lim, In-Bae Song, Youn-Hwan Hwang, Myoung-Seok Kim, Jong-Woo Kim, Jin-Yong Kim, Joo Won Suh, Hyo-In Yun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 2 115-120 (6 pages)
The isoflavonol glycoside Talosin A, genistein (GT)-7-a-L-6- deoxy talopyranose (GT-Tal), was first isolated from the culture broth of Kitasatospora kifunensis MJM341. The aim of the present study was to evaluate the oral absorption and metabolism of the newly isolated isoflavonol glycoside, GT-Tal compared to genistin (GT-7-O-b-D-glucopyranoside; GT- Glu). Free GT-Glu and GT-Tal could not be detected prior to enzymatic hydrolysis of the corresponding conjugates in rat plasma. Following oral... -
Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay
Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)
The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with novobiocin (MKTTn), on real-time PCR was confirmed. The inhibition of PCR was statistically significant (p < 0.05) in RV and MKTTn, as compared with buffered peptone water (BPW) or phosphate-buffered... -
PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases
Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 8 151-154 (4 pages)
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc),... -
Polysaccharides isolated from Phellinus gilvus enhances dermal wound healing in streptozotocin-induced diabetic rats
Jae-Sung Bae, Kwang-Ho Jang, Hee-Kyung Jin 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 12 161-164 (4 pages)
Dermal wound healing is a complex process that involved inflammation leading to re-epithelialization, granulation tissue, and tissue remodeling. Previous studies from our laboratory have shown that polysaccharides isolated from fungus, Phellinus gilvus (PG) have various anti-inflammatory activities. In present study, we have assessed the effect of polysaccharides from PG on the dermal wound healing of polysaccharides from PG in streptozotocin-induced diabetic rat model. Six of 6-mm circular... -
Histochemical Detection of Glycoconjugates in the Male Reproductive System of the Horse
Tae-young Ha, Mee-jung Ahn, Yong-duk Lee, Jae-hyuk Yang, Hee-seok Kim, Tae-kyun Shin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 4 21-28 (8 pages)
Lectins are glycoproteins of plant and animal origin that have the ability to bind specific carbohydrate residues of cell glycoconjugates, particularly in terminal positions. In this study, the binding of lectins, Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), Bandeiraea simplicifolia BS-1 (isolectin B4), Triticum vulgaris (WGA), Arachis hypogaea (PNA), and Ulex europaeus (UEA-I), was studied in the reproductive systems of male thoroughbred horses.DBA was detected in the... -
Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ
Jee-young Lee, Tae-young Kang, Yong-duk Lee, Tae-kyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 3 15-19 (5 pages)
The binding specificities of various lectins, such as the Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), and the Bandeiraea simplicifolia BS-1 (Isolectin B4), Triticum vulgaris (WGA), Arachis hypogaea (PNA), and Ulex europaeus (UEA-I) lectins, were studied in the vomeronasal organ of the horse. The microvilli of the vomeronasal sensory epithelium were positive for DBA, SBA, Isolectin B4, WGA, PNA, and UEA-I. The receptor cells showed intense reactivity for DBA and WGA. Lectins... -
Histochemistry of Six Lectins in the Tissues of the Flat Fish Paralichthys olivaceus
Kyung-Sook Jung, Mee-Jung Ahn, Yong-Duk Lee, Gyung-Min Go and Tae-Kyun Shin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 7 293-301 (9 pages)
Lectins are glycoproteins that specifically bind carbohydrate structures and may participate in the biodefense mechanisms of fish. In this study, the binding of three lectins, Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), Bandeiraea simplicifolia BS-1 (isolectin B4), Triticum vulgaris (WGA), Arachis hypogaea (PNA) and Ulex europaeus (UEA-I) were studied in the gill, liver, intestine, kidney, heart, and spleen of the flat fish Paralichthys olivaceus. DBA was detected in intestinal...


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