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Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR
Khalid Munir 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 13 167-176 (10 pages)
We have previously described the development of a onetube SYBR Green real-time RT-PCR assay for the detection and quantitation of infectious salmon anemia virus (ISAV) in various biological samples. The twofold aim of the present study was to verify that the optimized SYBR Green real-time RT-PCR conditions could detect ISAV isolates of different geographic origins, and to analyze the growth patterns of the selected ISAV isolates in the Chinook head salmon embryo (CHSE) -214 cells by this assay... -
Concurrent response to challenge infection with Cryptosporidium parvum in immunosuppressed C57BL/6N mice
Chan-Gu Surl , Hyeon-Cheol Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 8 47-51 (5 pages)
't show any oocyst shedding and parasite colonization.Especially, oocyst shedding and parasite colonization from the mice infected with heat- killed oocysts were not detected.After challenge infection with C.parvum oocysts, however, these mice were shedding small numbers of oocysts and parasite colonization. Except normal control and uninfected groups, the antibody titers of other groups appear similar.Based on the fecal oocyst shedding, parasite colonization of ilea, and antibody titers in the... -
Detection of Bartonella species from ticks, mites and small mammals in Korea
Chul Min Kim, Ji Young Kim, Ying Hua Yi, Mi Jin Lee, Mae rim Cho, Devendra H. Shah, Terry A. Klein, Heung Chul Kim, Jin Won Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 11 327-334 (8 pages)
infections in ticks, mites and small mammals (rodents, insectivores and weasels) collected during 2001 through 2004, from various military installations and training sites in Korea, using PCR and sequence analysis of 16S rRNA, 23S rRNA and groEL heat shock protein genes. The prevalence of Bartonella spp. was 5.2% (n = 1, 305 sample pools) in ticks, 19.1% (n = 21) in mesostigmatid mites and 13.7% (n = 424 individuals) in small mammals. The prevalence within the family Ixodidae was, 4.4% (n = 1,... -
Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats
Hye-Jin Yang, Sang-Hyun Lee, Yong Jin, Jin-Hyang Choi, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 3 103-109 (7 pages)
The toxicity of acrylamide was evaluated through mutagenicity of Salmonella, chromosome aberration of Chinese hamster lung fibroblasts, micronucleus formation in mice and reproductive toxicity in rats. Based on Ames test, acrylamide showed mutagenic potency for strains TA98 and TA100. Moreover, both chromosomal aberration assay and micronucleus assay indicated that acrylamide might have genotoxic potency; the chromosomal aberration frequencies were observed to be proportional to acrylamide... -
Thickness of cumulus cell layer is a significant factor in meiotic competence of buffalo oocytes
Hassan M. Warriach, Kazim R. Chohan 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 10 247-251 (5 pages)
This study evaluated the meiotic competence of buffalo oocytes with different layers of cumulus cells. A total of 588 oocytes were collected from 775 ovaries averaging 0.78 oocytes per ovary. Oocytes with homogenous cytoplasm (n = 441) were selected for in vitro maturation (IVM) and divided into four groups based on their cumulus morphology: a) oocytes with ≥= 3 layers of cumulus cells, b) 1-2 layers of cumulus cells and oocytes with partial remnants or no cumulus cells to be cocultured c) with... -
Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea
Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 6 125-130 (6 pages)
The isolate was further characterized by immuno-fluorescence assay using Japanese encephalitis virus (JEV) specific monoclonal antibodies. Reverse transcription polymerase chain reaction (RT-PCR) revealed the presence of JE specific conserved sequences in this isolate. The artificially inoculated pigs had HI titer of 320 to 2,560 against JEV at 14 to 42 days post inoculation. We confirmed this isolate as Japanese encephalitis virus. It was the second isolation of JEV in pigs in Korea. -
Inherited canine copper toxicosis in Australian Bedlington Terriers
Changbaig Hyun and Lucio John Filippich 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 4 19-28 (10 pages)
Inherited copper toxicosis in Bedlington Terriers (CTBT) is a copper associated hepatopathy caused by an autosomal recessive genetic defect of gene involving copper metabolism. To compare clinical and histopathological findings with previous reports and to expand our knowledge for future genetic studies, 18 terriers were clinically and histopathologically examined in this study. Pedigree information and dietary history were obtained from the owners before a thorough clinical examination was... -
Frequencies of Micronuclei in Peripheral Lymphocytes in Korean Populations after Chronic Low-dose Radiation Exposure
Mi-Young An and Tae-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 11 213-218 (6 pages)
The purpose of this study was to estimate predictive markers of intrinsic radiosensitivity in individuals who were exposed to occupational or environmental radiation. Throughout this process, the actual biohazard risks and base-line chromosome damage were evaluated in human population. Further studies were carried out to provide evidence for the existence of individual variations in age-dependent responses through micronuclei (MN) assay. Spontaneous frequencies not only vary greatly between... -
Biological Rhythm in Livestock
Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,... -
Localization of Porcine Reproductive and Respiratory Syndrome Virus Infection in Boars by In Situ Riboprobe Hybridization
Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 5 87-96 (10 pages)
The capability of porcine reproductive and respiratory syndrome virus (PRRSV) to be shed in semen for extended periods of time has been suggested to be a principal factor for viral transmission via insemination. In attempts to gain insights into the mechanism of PRRSV persistence in boars, tissue distribution and sites of viral infection were investigated by in situ hybridization (ISH) using digoxigenin-labeled RNA probe and the ISH results were compared with those of reverse...


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