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1431.

Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 저
...
- 3,000원
(0 to 1,000 mg/kg) ranged from 68.34 to 95.49% (CV; 4.10 to 13.38%). DON in a buffer solution (250, 500 and 1,000 ng/mL) was isolated using 300 μg of NVRQS-DON and 3 mg of magnetic nanoparticles (MNPs). The mean recovery rates of DON using this mAb-MNP system were 75.2, 96.9, and 88.1% in a buffer solution spiked with DON (250, 500, and 1,000 ng/mL). Conclusively we developed competitive ELISAs for detecting DON in animal feed and created a new tool for DON extraction using mAb-coupled MNPs.
1432.

Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry

AMAM Zonaed Siddiki* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 1 107-114 (8 pages)

AMAM Zonaed Siddiki* 저
...
- 3,000원
with subsequent LC-MS/MS analysis. A total of 135 protein hits were recorded from 20 gel slices (from same gel lane), with many hits occurring in more than one band. Excluding all non-Cryptosporidium entries and proteins with multiple hits, 33 separate C. parvum entries were identified during the study. The overall goal of this study was to reduce sample complexity by protein fractionation and increase the possibility of detecting proteins present in lower abundance in a complex protein mixture.
1433.

Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 저
...
- 3,000원
strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clone revealed a single 802-bp open reading frame encoding a partial protein with a...
1434.

C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

Kana Tozaki, Junpei Kimura, Masahiro Yasuda*, Nobuyuki Ryu, Testuo Nasu, Anton Pernthaner, Wayne R. Hein 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 1 1-6 (6 pages)

Kana Tozaki, Junpei Kimura, Masahiro Yasuda*, Nobuyuki Ryu, Testuo Nasu, Anton Pernthaner, Wayne R. Hein 저
...
- 3,000원
cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.
1435.

Molecular characterization of the feline T-cell receptor γ alternate reading frame protein (TARP) ortholog

Alexander Th. A. Weiss*, Marie-Charlotte von Deetzen, Werner Hecht, Manfred Reinacher, Achim D. Gruber 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 3 345-353 (9 pages)

Alexander Th. A. Weiss*, Marie-Charlotte von Deetzen, Werner Hecht, Manfred Reinacher, Achim D. Gruber 저
...
- 3,000원
but is not found in normal mammary tissue. To date, this protein has only been described in humans. Additionally, no animal model has been established to investigate the potential merits of TARP as tumor marker or a target for adoptive tumor immunotherapy. In this study conducted to characterize feline T-cell receptor γ sequences, constructs very similar to human TARP transcripts were obtained by RACE from the spleen and prostate gland of cats. Transcription of TARP in normal, hyperplastic, and...
1436.

Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Pam Dachung Luka*, Chrisostom Ayebazibwe, David Shamaki, Frank Norbert Mwiine, Joseph Erume 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 15 323-325 (3 pages)

Pam Dachung Luka*, Chrisostom Ayebazibwe, David Shamaki, Frank Norbert Mwiine, Joseph Erume 저
...
- 1,500원
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
1437.

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 저
...
- 3,000원
store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the cryopreservation procedure might also be error-prone. However, only limited amounts of material are available for analysis. To improve the monitoring of frozen/thawed spermatozoa, commonly used in vitro fertilization (IVF) followed by...
1438.

Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects

Byung-Jae Kang, Hak-Hyun Ryu, Sung Su Park, Yoshihisa Koyama, Masanori Kikuchi, Heung-Myong Woo, Wan Hee Kim, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 12 299-310 (12 pages)

Byung-Jae Kang, Hak-Hyun Ryu, Sung Su Park, Yoshihisa Koyama, Masanori Kikuchi, Heung-Myong Woo, Wan Hee Kim, Oh-Kyeong Kweon* 저
...
- 4,100원
of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field of bone tissue engineering. The purpose of this study was to compare the osteogenic potential of canine MSCs derived from adipose tissue (AT), BM, umbilical cord blood (UCB), and Wharton's jelly (WJ) using in vitro culture techniques and in vivo orthotopic implantation assays. After canine MSCs were isolated from various tissues, the proliferation and osteogenic potential along with...
1439.

Activation of Akt/protein kinase B mediates the protective effects of mechanical stretching against myocardial ischemia-reperfusion injury

Chan-Hyung Kim*, Jia Hao, Hee-Yul Ahn, Si Wook Kim* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 4 235-244 (10 pages)

Chan-Hyung Kim*, Jia Hao, Hee-Yul Ahn, Si Wook Kim* 저
...
- 3,000원
of Akt and GSK-3b. Furthermore, SIC was abrogated by wortmannin and Gd. In vivo stretching induced by an aorto-caval shunt increased Akt phosphorylation and reduced myocardial infarction; these effects were diminished by wortmannin and Gd pretreatment. Our results showed that mechanical stretching can provide cardioprotection against ischemia- reperfusion injury. Additionally, the activation of Akt, which might be regulated by SACs and the PI3K pathway, plays an important role in SIC.
1440.

Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions

Adriana Hamond Regua-Mangia*, Alice Gon?alves M. Gonzalez, Aloysio M. F. Cerqueira, Joao Ramos C. Andrade 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 4 139-144 (6 pages)

Adriana Hamond Regua-Mangia*, Alice Gon?alves M. Gonzalez, Aloysio M. F. Cerqueira, Joao Ramos C. Andrade 저
...
- 3,000원
the clonal structure. O157:H7 strains from several countries were isolated from food, human and bovine feces. According to MLEE, O157:H7 strains clustered into two main clonal groups designated A and B. Subcluster A1 included 82% of the O157:H7 strains exhibiting identical MLEE pattern. Most enterohemorrhagic E. coli (EHEC) O157:H7 strains from Brazil and Argentina were in the same MLEE subgroup. Bovine and food strains carried virulence genes associated with EHEC pathogenicity in humans.

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