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141.

Identification and characterization of atypical porcine pestivirus genomes in newborn piglets with congenital tremor in China

Hanqin Shen, Xiangcong Liu, Pengfei Zhang, Lei Wang, Yanling Liu, Leyi Zhang, Pengshuai Liang, Changxu Song 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 17 468-471 (4 pages)

Hanqin Shen, Xiangcong Liu, Pengfei Zhang, Lei Wang, Yanling Liu, Leyi Zhang, Pengshuai Liang, Changxu Song 저
...
- 1,500원
Recently, a novel atypical porcine pestivirus (APPV) in pig was reported. In this study, two APPV strains, APPV-China/GZ01/2016 (GZ01) and APPV-China/GD-SD/2016 (GD-SD), were identified in two newborn piglet herds with congenital tremor from China. The open reading frame of the two strains shared an 83.5% nucleotide identity. Phylogenetically, the APPV strains were placed into two groups: GZ01 belonged to group I and GD-SD belonged to group II. A high viral load was detected in the cerebellum...
142.

Production of transgenic pigs using a pGFAP-CreERT2/EGFP LoxP inducible system for central nervous system disease models

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 13 434-445 (12 pages)

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 저
...
- 4,100원
piglets at 115 days after pregnancy. Polymerase chain reaction (PCR) analysis with genomic DNA isolated from skin tissues of TG pigs revealed that all 5 TG pigs had the transgenes. EGFP expression in all organs tested was confirmed by immunofluorescence staining and PCR. Real-time PCR analysis showed that pGFAP promoter-driven Cre fused to the mutated human ligand-binding domain of the estrogen receptor (CreERT2) mRNA was highly expressed in the cerebrum. Semi-nested PCR analysis revealed that...
143.

Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine

Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 11 416-425 (10 pages)

Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee 저
...
- 3,000원
An anti-Brucella vaccine candidate comprised of purified Brucella lipopolysaccharide (LPS) and a cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported previously. Each vector constitutively expressed highly conserved Brucella antigens (rB), viz., lumazine synthase (BLS), proline racemase subunit A, outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined a relative level of protection conferred by each single strain. Upon virulent...
144.

Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 저
...
- 3,000원
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain...
145.

Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 3 339-349 (11 pages)

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 저
...
- 4,000원
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple...
146.

Haemoproteus in barn and collared scops owls from Thailand

Chaleow Salakij, Pornchai Pornpanom, Preeda Lertwatcharasarakul, Chaiyan Kasorndorkbua, Jarernsak Salakij 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 14 280-289 (10 pages)

Chaleow Salakij, Pornchai Pornpanom, Preeda Lertwatcharasarakul, Chaiyan Kasorndorkbua, Jarernsak Salakij 저
...
- 3,000원
The barn owl (BO) and the collared scops owl (CSO) are common nocturnal raptors throughout Thailand. Blood samples from 23 adult BOs and 14 CSOs were collected and processed for complete blood cell counts and parasite morphological examinations. Two Haemoproteus-positive samples were processed for ultrastructural observation. Polymerase chain reaction (PCR) analysis for a partial cytochrome b gene (cytb) from Haemoproteus was performed in all samples. Haemoproteus presence detected by light...
147.

A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer

Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)

Qing Wang, Mianmian Chen, Wei Zhang 저
...
- 4,000원
Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction...
148.

Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick

Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 10 242-250 (9 pages)

Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 저
...
- 3,000원
testing is infrequent due to the lack of a robust method. The isothermal recombinase polymerase amplification (RPA) technique has applied for rapid diagnosis. Herein, RPA combined with a lateral flow dipstick (LFD) assay was developed to estimate DNA from Mycobacterium avium subsp. paratuberculosis. First, analytical specificity and sensitivity of the RPA-nfo primer and probe sets were assessed. The assay successfully detected M. paratuberculosis DNA in 30 min at 39°C with a detection limit of...
149.

Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

Ke Ding, Ke Shang, Zu-Hua Yu, Chuan Yu, Yan-Yan Jia, Lei He, Cheng-Shui Liao, Jing Li, Chun-Jie Zhang, Yin-Ju Li, Ting-Cai Wu, X 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 9 232-241 (10 pages)

Ke Ding, Ke Shang, Zu-Hua Yu, Chuan Yu, Yan-Yan Jia, Lei He, Cheng-Shui Liao, Jing Li, Chun-Jie Zhang, Yin-Ju Li, Ting-Cai Wu, X 저
...
- 3,000원
Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccine can protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologous recombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S....
150.

Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method

Yuetao Li, Yongkun Zhao, Cuiling Wang, Xuexing Zheng, Hualei Wang, Weiwei Gai, Hongli Jin, Feihu Yan, Boning Qiu, Yuwei Gao, Nan 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 6 200-206 (7 pages)

Yuetao Li, Yongkun Zhao, Cuiling Wang, Xuexing Zheng, Hualei Wang, Weiwei Gai, Hongli Jin, Feihu Yan, Boning Qiu, Yuwei Gao, Nan 저
...
- 3,000원
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV...

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