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141.

Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 저
...
- 3,000원
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated...
142.

Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats

Hyo-jin Chung, Wook-hun Chung, Jae-Hoon Lee, Dai-Jung Chung, Wo-Jong Yang, A-Jin Lee, Chi-Bong Choi, Hwa-Seok Chang, Dae-Hyun Ki 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 12 97-102 (6 pages)

Hyo-jin Chung, Wook-hun Chung, Jae-Hoon Lee, Dai-Jung Chung, Wo-Jong Yang, A-Jin Lee, Chi-Bong Choi, Hwa-Seok Chang, Dae-Hyun Ki 저
...
- 3,000원
We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI...
143.

Production of α 1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology

Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 11 89-96 (8 pages)

Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 저
...
- 3,000원
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation....
144.

Gap junction blockage promotes cadmium-induced apoptosis in BRL 3A derived from Buffalo rat liver cells

Di Hu, Hui Zou, Tao Han, Junze Xie, Nannan Dai, Liling Zhuo, Jianhong Gu, Jianchun Bian, Yan Yuan, Xuezhong Liu, Zongping Liu 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 8 63-70 (8 pages)

Di Hu, Hui Zou, Tao Han, Junze Xie, Nannan Dai, Liling Zhuo, Jianhong Gu, Jianchun Bian, Yan Yuan, Xuezhong Liu, Zongping Liu 저
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- 3,000원
Gap junctions mediate direct communication between cells; however, toxicological cascade triggered by nonessential metals can abrogate cellular signaling mediated by gap junctions. Although cadmium (Cd) is known to induce apoptosis in organs and tissues, the mechanisms that underlie gap junction activity in Cd-induced apoptosis in BRL 3A rat liver cells has yet to be established. In this study, we showed that Cd treatment decreased the cell index (a measure of cellular electrical impedance) in...
145.

Assessment of the safety and efficacy of low pathogenic avian influenza (H9N2) virus in inactivated oil emulsion vaccine in laying hens

Jeong-Hwa Shin, Jong Seo Mo, Jong-Nyeo Kim, In-pil Mo, Bong-Do Ha 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 4 27-34 (8 pages)

Jeong-Hwa Shin, Jong Seo Mo, Jong-Nyeo Kim, In-pil Mo, Bong-Do Ha 저
...
- 3,000원
In Korea, several outbreaks of low pathogenic AI (H9N2) viral infections leading to decreased egg production and increased mortality have been reported on commercial farms since 1996, resulting in severe economic losses. To control the H9N2 LPAI endemic, the Korea Veterinary Authority has permitted the use of the inactivated H9N2 LPAI vaccine since 2007. In this study, we developed a killed vaccine using a low pathogenic H9N2 AI virus (A/chicken/Korea/ADL0401) and conducted safety and efficacy...
146.

Hepatitis E virus as an emerging zoonotic pathogen

Woo-Jung Park, Byung-Joo Park, Hee-Seop Ahn, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Han-Sang Yoo, In-Soo 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 1 1-11 (11 pages)

Woo-Jung Park, Byung-Joo Park, Hee-Seop Ahn, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Han-Sang Yoo, In-Soo 저
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- 4,000원
Hepatitis E outbreaks are a serious public health concern in developing countries. The disease causes acute infections, primarily in young adults. The mortality rate is approximately 2%; however, it can exceed 20% in pregnant women in some regions in India. The causative agent, hepatitis E virus (HEV), has been isolated from several animal species, including pigs. HEV genotypes 3 and 4 have been isolated from both humans and animals, and are recognized as zoonotic pathogens. Seroprevalence...
147.

Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV

Kiarash Roohani, Sheau Wei Tan, Swee Keong Yeap, Aini Ideris, Mohd Hair Bejo, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 8 447-457 (11 pages)

Kiarash Roohani, Sheau Wei Tan, Swee Keong Yeap, Aini Ideris, Mohd Hair Bejo, Abdul Rahman Omar 저
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- 4,000원
A Newcastle disease virus (NDV) isolate designated IBS002 was isolated from a commercial broiler farm in Malaysia. The virus was characterised as a virulent strain based on the multiple basic amino acid motif of the fusion (F) cleavage site RRRKGF and length of the C-terminus extension of the hemagglutinin-neuraminidase (HN) gene. Furthermore, IBS002 was classified as a velogenic NDV with mean death time (MDT) of 51.2 h and intracerebral pathogenicity index (ICPI) of 1.76. A genetic distance...
148.

Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 저
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- 3,000원
Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the...
149.

Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China from 2012 to 2013

Han-Qin Shen, Zhuan-Qiang Yan, Fan-Gui Zeng, Chang-Tao Liao, Qing-Feng Zhou, Jian-Ping Qin, Qing-Mei Xie, Ying-Zuo Bi, Feng Chen 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 9 317-324 (8 pages)

Han-Qin Shen, Zhuan-Qiang Yan, Fan-Gui Zeng, Chang-Tao Liao, Qing-Feng Zhou, Jian-Ping Qin, Qing-Mei Xie, Ying-Zuo Bi, Feng Chen 저
...
- 3,000원
As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) were isolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes of these strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, while the other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses...
150.

Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus

Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)

Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 저
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- 3,000원
Integrin ανβ3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of ανβ3 integrin, a stable CHO-677 cell line expressing the murine ανβ3 heterodimer (designated as “CHO-677-mανβ3” cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect...

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