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141.

Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field

B. Adil, K. M. Shankar, B. T. Naveen Kumar, Rajreddy Patil, Abhiman Ballyaya, K. S. Ramesh, Sathish Rama Poojary, Omkar V. Byadg 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 6 413-419 (7 pages)

B. Adil, K. M. Shankar, B. T. Naveen Kumar, Rajreddy Patil, Abhiman Ballyaya, K. S. Ramesh, Sathish Rama Poojary, Omkar V. Byadg 저
...
- 3,000원
A monoclonal antibody-based flow-through immunoassay (FTA) was developed using a nitrocellulose membrane placed on the top of adsorbent pads enclosed in a plastic cassette with a test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.) invadans. The FTA limit of detection was 7 μg/mL for A.invadans compared to 56 μg/mL for the immunodot. FTA and polymerase chain reaction (PCR) could detect...
142.

Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 5 263-270 (8 pages)

Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 저
...
- 3,000원
Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and...
143.

Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos

In-Sun Hwang, Hyo-Kyung Bae, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 1 235-240 (6 pages)

In-Sun Hwang, Hyo-Kyung Bae, Hee-Tae Cheong 저
...
- 3,000원
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ·OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were...
144.

Isolation and characterization of orf viruses from Korean black goats

Jae-Ku Oem, Joon-Yee Chung, Yong-Joo Kim, Kyoung-Ki Lee, Seong-Hee Kim, Byeong-Yeal Jung, Bang-Hun Hyun* 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 16 227-230 (4 pages)

Jae-Ku Oem, Joon-Yee Chung, Yong-Joo Kim, Kyoung-Ki Lee, Seong-Hee Kim, Byeong-Yeal Jung, Bang-Hun Hyun* 저
...
- 1,500원
Five cases of orf virus infection in Korean black goats were diagnosed in our laboratory between 2010 and 2011. One orf virus (ORF/2011) was isolated from an ovine testis cell line (OA3.Ts) for use as a vaccine candidate. Sequences of the major envelope protein and orf virus interferon resistance genes were determined and compared with published reference sequences. Phylogenetic analyses revealed that orf viruses from Korean black goats were most closely related to an isolate (ORF/09/Korea) from...
145.

Identification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR

Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 8 167-173 (7 pages)

Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 저
...
- 3,000원
Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment sizes of approximately 200 to 1,500 bp. The eight fragments were expressed differentially in the...
146.

Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea

Eun-Jin Choi, Chang-Hee Lee, Jae-Young Song, Hee-Jong Song, Choi-Kyu Park, Byounghan Kim, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 2 115-124 (10 pages)

Eun-Jin Choi, Chang-Hee Lee, Jae-Young Song, Hee-Jong Song, Choi-Kyu Park, Byounghan Kim, Yeun-Kyung Shin* 저
...
- 3,000원
The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms...
147.

Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

Fang Yan*, Yujun Zhao, Yongting Hu, Jianyang Qiu, Wenxin Lei, Wenhui Ji, Xuying Li, Qian Wu, Xiumin Shi, Zhong Li 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 8 53-60 (8 pages)

Fang Yan*, Yujun Zhao, Yongting Hu, Jianyang Qiu, Wenxin Lei, Wenhui Ji, Xuying Li, Qian Wu, Xiumin Shi, Zhong Li 저
...
- 3,000원
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody...
148.

Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 저
...
- 3,000원
Treatment of bovine mastitis caused by Staphylococcus (S.) aureus is becoming very difficult due to the emergence of multidrug-resistant strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence...
149.

Genetic populations of Bacillus anthracis isolates from Korea

Kyoung Hwa Jung, Sang Hoon Kim, Se Kye Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Ji Cheon Kim, Seoung Joo Kim, Hee-Bok 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 8 385-393 (9 pages)

Kyoung Hwa Jung, Sang Hoon Kim, Se Kye Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Ji Cheon Kim, Seoung Joo Kim, Hee-Bok 저
...
- 3,000원
Bacillus (B.) anthracis is the pathogen that causes fatal anthrax. Strain-specific detection of this bacterium using molecular approaches has enhanced our knowledge of microbial population genetics. In the present study, we employed molecular approaches including multiple-locus variable-number tandem repeat analysis (MLVA) and canonical single-nucleotide polymorphism (canSNP) analysis to perform molecular typing of B. anthracis strains isolated in Korea. According to the MLVA, 17 B. anthracis...
150.

A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea

Eun-Jin Choi, Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 7 377-383 (7 pages)

Eun-Jin Choi, Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin* 저
...
- 3,000원
No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU,...

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