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1491.

Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 9 452-459 (8 pages)

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- 3,000원
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate...
1492.

Epidemiology and Control of Strawberry Bacterial Angular Leaf Spot Disease Caused by Xanthomonas fragariae

한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 4호 2 290-299 (10 pages)

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- 3,000원
Strawberry bacterial angular leaf spot (ALS) disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small angular water-soaked lesions to develop on the abaxial leaf surface. Studies reported optimum temperature conditions for X. fragariae are 20°C and the pathogen suffers mortality above 32°C. However, at the nursery stage, disease symptoms have been observed under high temperature conditions. In the present...
1493.

Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 2 212-218 (7 pages)

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- 3,000원
In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a...
1494.

Note : Open Access ; Occurrence of Stolbur Phytoplasma Disease in Spreading Type Petunia hybrida Cultivars in Korea

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 15 465-470 (6 pages)

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- 3,000원
In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, and through sequencing 12 Pet-Stol isolates were identified. Comparison of the nucleotide sequences of...
1495.

Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 12 99-104 (6 pages)

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- 3,000원
1496.

Biological Efficacy of Streptomyces sp. Strain BN1 against the Cereal Head Blight Pathogen Fusarium graminearum

한국식물병리학회 The Plant Pathology Journal 15 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 6 52-66 (15 pages)

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- 4,400원
Fusarium head blight (FHB) caused by the filamentous fungus Fusarium graminearum is one of the most severe diseases threatening the production of small grains. Infected grains are often contaminated with mycotoxins such as zearalenone and trichothecences. During survey of contamination by FHB in rice grains, we found a bacterial isolate, designated as BN1, antagonistic to F. graminearum. The strain BN1 had branching vegetative hyphae and spores, and its aerial hyphae often had long, straight...
1497.

Research Articles : Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 2 310-314 (5 pages)

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- 1,500원
Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP...
1498.

Suppression of UDP-glycosyltransferase-coding Arabidopsis thaliana UGT74E2 Gene Expression Leads to Increased Resistance to Psuedomonas syringae pv. tomato DC3000 Infection

한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 10 170-182 (13 pages)

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- 4,200원
Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection...
1499.

Molecular Cloning and Characterization of Soybean Cinnamoyl CoA Reductase Induced by Abiotic Stresses

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 12 380-385 (6 pages)

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- 3,000원
Suppression subtractive hybridization was used to isolate wound-induced genes from soybean. One of the wound-induced genes, gmwi143 designated as GmCCR, showed high homology with genes encoding cinnamoyl- CoA reductase (CCR; EC 1.2.1.44). Deduced amino acid sequences encoded by GmCCR showed the highest identity (77%) with those of Acacia CCR. There are 2 CCR genes highly homologous to GmCCR in soybean genome based on Phytozome DB analysis. RNA expression of GmCCR was specifically induced by...
1500.

Potential of the Volatile-Producing Fungus Nodulisporium sp. CF016 for the Control of Postharvest Diseases of Apple

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 7 253-259 (7 pages)

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- 3,000원
In vitro and in vivo mycofumigation effects of the volatileproducing fungus Nodulisporium sp. CF016 isolated from stem of Cinnamomum loureirii and the role of its volatile compounds were investigated against phytopathogenic fungi. The volatile compounds produced by Nodulisporium sp. CF016 inhibited and killed a wide range of plant and storage pathogens including to Pythium ultimum, Rhizoctonia solani, Fusarium oxysporum, Phytophthora capsici, Sclerotinia sclerotiorum, Colletotrichum coccodes,...

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