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총 560건 검색
본문검색 결과만 보기
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Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine
Dong-Kun Yang, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Kyung-Woo Lee, Cheong-Up Choi, Chang-Hee Kweon 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 10 271-275 (5 pages)
The Japanese encephalitis virus (JEV) is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay (I-ELISA) was examined for its potential use in the rapid monitoring of the JEV, and the results were compared with those from the hemagglutination inhibition (HI) and serum neutralization (SN) tests. The comparative analysis showed that the results of I-ELISA showed a significant correlation with the conventional HI (r = 0.867) and SN tests (r... -
Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes
Jong Hyeon Park, Sun Jin Kim, Jae Ku Oem, Kwang Nyeong Lee, Yong Joo Kim, Soo Jeong Kye, Jee Yong Park, Yi Seok Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 8 257-262 (6 pages)
The capsid of the foot and mouth disease (FMD) virus carries the epitopes that are critical for inducing the immune response. In an attempt to enhance the specific immune response, plasmid DNA was constructed to express VP1/interleukin-1α (IL-1α) and precursor capsid (P1) in combination with 2A (P1-2A)/IL-1α under the control of the human cytomegalovirus (HCMV) immediateearly promoter and intron. After DNA transfection into MA104 (monkey kidney) cells, Western blotting and an... -
Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin
Sitarina Widyarini 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 2 217-223 (7 pages)
Equol, an isoflavonoid metabolite produced from the dietary isoflavone daidzein by the gut microflora in mammals, has been found to protect not only against ultraviolet (UV) radiation-induced cutaneous inflammation and photoimmune suppression, but also have antiphotocarcinogenic properties in mice. Because the state of DNA damage has been correlated with suppression of the immune system and photocarcinogenesis, we have therefore examined the potential of equol to offer protection from... -
Development of immunoassays for the detection of kanamycin in veterinary fields
Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 3 111-117 (7 pages)
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of... -
Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat
Young-Bae Kwon, Hyun-Woo Kim, Dae-Hyun Roh, Seo-Yeon Yoon, Rong-Min Baek, Jeum -Yong Kim, HaeYong Kweon, Kwang-Gill Lee, Young-H 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 2 105-109 (5 pages)
Recombinant human epidermal growth factor (rhEGF) stimulates the proliferation and migration of epithelial cells in human cell culture systems and animal models of partial-thickness skin wounds. This study investigated the effect of a topical rhEGF ointment on the rate of wound healing and skin re-epithelialization in a rat full thickness wound model, and verified whether or not the rhEGF treatment affected both myofibroblast proliferation and collagen synthesis in the dermis. When rhEGF (10... -
Concurrent response to challenge infection with Cryptosporidium parvum in immunosuppressed C57BL/6N mice
Chan-Gu Surl , Hyeon-Cheol Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 8 47-51 (5 pages)
We investigated the response to challenge infection with Cryptosporidium parvum oocysts in immunosuppressed C57BL/6N mice.In the primary infection, fecal oocyst shedding and parasite colonization were greater in immunosuppressed mice than in nonimmunosuppressed mice.Compared with primary infection, challenge infection with C.parvum didn 't show any oocyst shedding and parasite colonization.Especially, oocyst shedding and parasite colonization from the mice infected with heat- killed oocysts were... -
Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus
Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Jong Hyeon Park, Yong Joo Kim, Hee Jong Song, Max Yeh 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 10 317-325 (9 pages)
It was reported that the sera of convalescent animals contain antibodies to foot and mouth disease (FMD) virus (FMDV) 2C, highly conserved nonstructural protein (NSP), whereas the sera of vaccinated animals do not. But ELISA methods using this protein were not reported and developed until recently. In this study, NSP 2C peptides were synthesized within the amino acid sequence of the conserved 2C nonstructural region of FMDV according to the sequences from Genbank database and used for... -
Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat
Hyo Jung Kwon, Kyoung Youl Lee, Il Kwon Park, Mi Sun Park, Mi Young Lee, Moo Kang Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 2 185-189 (5 pages)
Tyrosine kinase A (TrkA)is an essential component of the high affinity nerve growth factor (NGF)receptor necessary to the mediate the biological effects of the neurotrophins, NGF. This study examined the distribution of TrkA-immunoreactivity (IR)cells in the postnatal rat cerebral cortex and the changes that occur in postnatal development as a result of the expression of this protein. TrkA-IR was detected at postnatal day (PD)3, PD6, PD9 and PD15. Base upon their somatodendritic morphology, the... -
Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes
Elizabeth C. Molina 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 7 135-139 (5 pages)
This study investigated the presence of cytokines interferon (IFN)-gamma, interleukins (IL) -6 and -8 in serum of cattle and buffaloes infected with Fasciola gigantica from one to 16 weeks post-infection to determine their T cell response during infection. The concentration of these cytokines was determined by sandwich enzyme-linked immunosorbent assay (ELISA). No IFN-gamma was detected in these animals while IL-6 was elevated from one to 16 weeks postinfection. Levels of IL-8 were also elevated... -
Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins...
