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Specific PCR Detection of Four Quarantine Fusarium Species in Korea
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 16 409-416 (8 pages)
Fusarium species, a large group of plant pathogens, potentially pose quarantine concerns worldwide. Here, we focus on the development of a method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer... -
Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 10 267-270 (4 pages)
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. -
Development of Recombinant Coat Protein Antibody Based IC-RT-PCR and Comparison of its Sensitivity with Other Immunoassays for the Detection of Papaya Ringspot Virus Isolates from India
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 4 25-31 (7 pages)
Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector, sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein... -
Development of Diagnostic Technology of Xylella fastidiosa Using Loop-Mediated Isothermal Amplification and PCR Methods
Suyoung Kim, Yujin Park, Gidon Kim 한국식물병리학회 식물병연구 7 Pages
한국식물병리학회 식물병연구 2021, 27권 1호 6 38-44 (7 pages)
Xylella fastidiosa is the most damaging pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for speci- ficity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four... -
Extracellular Polymeric Substances of Pseudomonas chlororaphis O6 Induce Systemic Drought Tolerance in Plants
Song Mi Cho, Anne J. Anderson, Young Cheol Kim 한국식물병리학회 식물병연구 6 Pages
한국식물병리학회 식물병연구 2018, 24권 3호 9 242-247 (6 pages)
of Arabidopsis thaliana also reduce the size of stomatal openings. Growth of A. thaliana seedlings with applications of the EPS from P. chlororaphis O6 reduced the extent of wilting when water was withheld from the plants. Fluorescence measurements showed photosystem II was protected in the A. thaliana leaves in the water stressed EPS-exposed plants. These findings indicate that P. chlororaphis O6 has redundancy in traits associated with induction of mechanisms to limit water stress in plants. -
남자 중학생의 성역할갈등과 진로장벽 간의 관계에서 진로결정자기효능감의 매개효과
정미나, 정구일 한국청소년상담학회 한국청소년상담학회지 21 Pages
한국청소년상담학회 한국청소년상담학회지 2018, 제 3권 제 2호 1 1-21 (21 pages)
본 연구의 목적은 남자 중학생을 대상으로 성역할갈등과 진로장벽 간의 관계를 알아보고, 성역할갈등과 진로장벽 간의 관계에서 진로결정자기효능감이 매개역할을 하는지 확인하고자 하였다. 본 연구는 서울시 소재 남자 중학생 150명의 자료를 최종 분석하였다. 본 연구결과를 요약하면 다음과 같다. 첫째, 주요 변인 간의 상관관계를 보면, 성역할갈등은 진로장벽과 유의미한 정적 상관을 보였고, 진로결정자기효능감과는 유의미한 부적 상관을 보였다. 진로결정자기효능은 진로장벽과 유의미한 부적 상관을 보였다. 둘째, 성역할... -
Effects of Long- and Short-term Consumption of Energy Drinks on Anxiety-like, Depression-like, and Cognitive Behavior in Adolescent Rats
Lee Joo Hee, Lee Jong Hyeon, Choi You Jeong, Kim Youn Jung 한국기초간호학회 Journal of Korean Biological Nursing Science 8 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2020, Volume 22, No 2 5 111-118 (8 pages)
Purpose: The purpose of this study was to understand the impact of long- and short-term energy drinks on anxiety-like, depressionlike, and cognitive behavior in adolescent rats. Methods: Adolescent rats (age six weeks) were randomly classified into a control group (CON), a long-term administration group (LT), and a short-term administration group (ST). The LT group was orally administered 1.5 mL/100 g (body weight) of energy drink twice daily for 14 days, the ST group was orally administered for... -
한국형 DASH 교육 프로그램이 여성노인의 산화스트레스, 항산화능력과 호모시스테인 농도에 미치는 효과
최승혜, 최스미, 곽충실, 이해영 한국기초간호학회 Journal of Korean Biological Nursing Science 10 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2017, Volume 19, No 3 3 141-150 (10 pages)
노화과정은 활성산소(reactive oxygen species, ROS)와 우리 체내 항산화 능력 간의 불균형으로 인한 산화스트레스(oxidative stress)와 밀접한 관련이 있는 것으로 알려져 있다[1]. 산화스트레스는 심혈관계 질환, 호흡기, 근골격계 질환 같은 노인성 질환과 수명에 영향을 미친다[1,2]. 산화스트레스는 다양한 요인에 의해 발생하는 것으로 보고되었으며[1,2], 그 중 호모시스테인은 산화과정을 통해 동맥혈관에 급격한 산화스트레스를 유발하여[3] 동맥경화증의 독립적인 위험인자로 알려져 있다.
