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161.

Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Pam Dachung Luka*, Chrisostom Ayebazibwe, David Shamaki, Frank Norbert Mwiine, Joseph Erume 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 15 323-325 (3 pages)

Pam Dachung Luka*, Chrisostom Ayebazibwe, David Shamaki, Frank Norbert Mwiine, Joseph Erume 저
...
- 1,500원
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
162.

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 저
...
- 3,000원
Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the...
163.

Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV

Vinayagamurthy Balamurugan, Arnab Sen, Gnanavel Venkatesan, Vandana Bhanot, Vineeta Yadav, Veerakyathappa Bhanuprakash, Raj Kuma 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 13 203-206 (4 pages)

Vinayagamurthy Balamurugan, Arnab Sen, Gnanavel Venkatesan, Vandana Bhanot, Vineeta Yadav, Veerakyathappa Bhanuprakash, Raj Kuma 저
...
- 1,500원
In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and...
164.

Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

Sirin Theerawatanasirikul, Gunnaporn Suriyaphol, Roongroje Thanawongnuwech, Achariya Sailasuta* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 7 163-170 (8 pages)

Sirin Theerawatanasirikul, Gunnaporn Suriyaphol, Roongroje Thanawongnuwech, Achariya Sailasuta* 저
...
- 3,000원
The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5), and their mRNA expression. This was done in the skin of five breeds of dogs including seven poodles, six golden retrievers, six Shih Tzus, four pugs, and four Labrador retrievers. Epidermal thickness of the stratum corneum and nucleated epidermal layer was significantly different. The greatest...
165.

Pathotyping avian pathogenic Escherichia coli strains in Korea

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 5 145-152 (8 pages)

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 저
...
- 3,000원
To examine the genetic background of avian pathogenic Escherichia coli (APEC) that affects virulence of this microorganism, we characterized the virulence genes of 101 APEC strains isolated from infected chickens between 1985∼2005. Serotypes were determined with available anti-sera and median lethal doses were determined in subcutaneously inoculated chicks. The virulence genes we tested included ones encoding type 1 fimbriae (fimC), iron uptake-related (iroN, irp2, iucD, and fyuA), toxins (lt,...
166.

Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain

Gil-Soon Park, Seong-In Lim, Seung-Ho Hong, Jae-Young Song* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 12 81-91 (11 pages)

Gil-Soon Park, Seong-In Lim, Seung-Ho Hong, Jae-Young Song* 저
...
- 4,000원
Classical swine fever virus (CSFV) causes a highly contagious disease among swine that has an important economic impact worldwide. CSFV strain LOM is an attenuated virus of low virulent strain of Miyagi isolated from Japan in 1956. Eight DNA fragments representing the genome of the CSFV strain LOM were obtained by RT-PCR. These were used to determine the complete nucleotide sequence and construct a full-length cDNA clone which was called Flc-LOM. Sequence analysis of the recombinant clone...
167.

Fast neutron irradiation deteriorates hippocampus-related memory ability in adult mice

Miyoung Yang, Hwanseong Kim, Juhwan Kim, Sung-Ho Kim, Jong-Choon Kim, Chun-Sik Bae, Joong-Sun Kim, Taekyun Shin, Changjong Moon* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 1 1-6 (6 pages)

Miyoung Yang, Hwanseong Kim, Juhwan Kim, Sung-Ho Kim, Jong-Choon Kim, Chun-Sik Bae, Joong-Sun Kim, Taekyun Shin, Changjong Moon* 저
...
- 3,000원
Object recognition memory and contextual fear conditioning task performance in adult C57BL/6 mice exposed to cranial fast neutron irradiation (0.8 Gy) were examined to evaluate hippocampus-related behavioral dysfunction following acute exposure to relatively low doses of fast neutrons. In addition, hippocampal neurogenesis changes in adult murine brain after cranial irradiation were analyzed using the neurogenesis immunohistochemical markers Ki-67 and doublecortin (DCX). In the object...
168.

Post-mortem re-cloning of a transgenic red fluorescent protein dog

So Gun Hong, Ok Jae Koo, Hyun Ju Oh, Jung Eun Park, Minjung Kim, Geon-A Kim, Eun Jung Park, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 15 405-407 (3 pages)

So Gun Hong, Ok Jae Koo, Hyun Ju Oh, Jung Eun Park, Minjung Kim, Geon-A Kim, Eun Jung Park, Goo Jang, Byeong-Chun Lee* 저
...
- 1,500원
obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
169.

Genotypic characterization of Staphylococcus aureus isolated from bovines, humans, and food in Indonesia

Siti Isrina Oktavia Salasia*, Syarifudin Tato, Ngalijan Sugiyono, Dwi Ariyanti, Feny Prabawati 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 8 353-361 (9 pages)

Siti Isrina Oktavia Salasia*, Syarifudin Tato, Ngalijan Sugiyono, Dwi Ariyanti, Feny Prabawati 저
...
- 3,000원
The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated the prevalence of staphylococcal enterotoxin (SE) genes and other virulence gene determinants by PCR. The identification of S. aureus was based on culturing and biochemical tests, and by amplifying a specific section of the 23S rRNA gene. PCR amplification of the SE genes...
170.

Apoptosis induced in vivo by new type gosling viral enteritis virus

Shun Chen, Anchun Cheng*, Mingshu Wang*, Dekang Zhu, Renyong Jia, Qihui Luo, Hengmin Cui, Yi Zhou, Yin Wang, Zhiwen Xu, Zhengli 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 5 333-339 (7 pages)

Shun Chen, Anchun Cheng*, Mingshu Wang*, Dekang Zhu, Renyong Jia, Qihui Luo, Hengmin Cui, Yi Zhou, Yin Wang, Zhiwen Xu, Zhengli 저
...
- 3,000원
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV- CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy....

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