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Experimental infection and pathology of clade 2.2 H5N1 virus in gulls
Marina A. Gulyaeva, Kirill A. Sharshov, Anna V. Zaykovskaia, Lidia V. Shestopalova, Aleksander M. Shestopalov 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 7 179-188 (10 pages)
During 2006, H5N1 HPAI caused an epizootic in wild birds, resulting in a die-off of Laridae in the Novosibirsk region at Chany Lake. In the present study, we infected common gulls (Larus canus) with a high dose of the H5N1 HPAI virus isolated from a common gull to determine if severe disease could be induced over the 28 day experimental period. Moderate clinical signs including diarrhea, conjunctivitis, respiratory distress and neurological signs were observed in virus-inoculated birds, and 50%... -
Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
Cellular prion protein (PrP) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting... -
Differential regulation of senescence and in vitro differentiation by 17β-estradiol between mesenchymal stem cells derived from male and female mini-pigs
Won-Jae Lee, Seung-Chan Lee, Jeong-Hyun Lee, Gyu-Jin Rho, Sung-Lim Lee 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 5 159-170 (12 pages)
The characterization and potential of mesenchymal stem cells (MSCs) are gender dependent and estrogen influences these properties. This study demonstrated that supplementation with 17β-estradiol (E2) increases the proliferation of bone marrow-MSCs derived from male and female mini-pigs (Mp- and Fp-BMSCs) in a concentration-dependent manner, with 10 M E2 suggested as the optimal dose of E2 that led to the greatest improvement in BMSCs proliferation. Supplementation of 10 M E2 resulted in... -
Time-course changes of hippocalcin expression in the mouse hippocampus following pilocarpine-induced status epilepticus
Hee-Soo Choi, Choong-Hyun Lee* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 2 137-144 (8 pages)
Hippocalcin participates in the maintenance of neuronal calcium homeostasis. In the present study, we examined the time-course changes of neuronal degeneration and hippocalcin protein level in the mouse hippocampus following pilocarpine-induced status epilepticus (SE). Marked neuronal degeneration was observed in the hippocampus after SE in a time-dependent manner, although neuronal degeneration differed according to the hippocampal subregions. Almost no hippocalcin immunoreactivity was detected... -
Effects of aluminum on the reduction of neural stem cells, proliferating cells, and differentiating neuroblasts in the dentate gyrus of D-galactose-treated mice via increasing oxidative stress
Sung Min Nam, Jong Whi Kim, Dae Young Yoo, Woosuk Kim, Hyo Young Jung, Jung Hoon Choi, In Koo Hwang, Je Kyung Seong, Yeo Sung Yo 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 1 127-136 (10 pages)
reduction effect was exacerbated in D-galactose-treated mice compared to vehicle-treated adult mice. Moreover, exposure to Al enhanced lipid peroxidation in the hippocampus and expression of antioxidants such as Cu, Zn- and Mn-superoxide dismutase in D-galactose-treated mice. These results suggest that Al accelerates the reduction of neural stem cells, proliferating cells, and differentiating neuroblasts in D-galactose-treated mice via oxidative stress, without inducing loss in mature neurons. -
Transplantation of adipose derived mesenchymal stem cells for acute thoracolumbar disc disease with no deep pain perception in dogs
Yongsun Kim, Seung Hoon Lee, Wan Hee Kim, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 17 123-126 (4 pages)
Thirty-four dogs with no deep pain perception due to acute thoracolumbar intervertebral disc disease underwent decompression surgery within 1 week of diagnosis. All dogs underwent hemilaminectomy. Adipose derived mesenchymal stem cells (AD-MSCs) were transplanted into the injured spinal cord parenchyma for the AD-MSCs transplant dogs. Long-term outcome was evaluated at the end of the follow-up period (> 6 months). AD-MSCs combination treatment showed better recovery outcomes compared to... -
Time-dependent low-field MRI characteristics of canine blood: an in vitro study
Jimo Jeong, Sangjun Park, Eunseok Jeong, Namsoo Kim, Minsu Kim, Yechan Jung, Youngkwon Cho, Kichang Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 13 103-109 (7 pages)
This study was conducted to assess time-sensitive magnetic resonance (MR) changes in canine blood using low-field MR. Arterial and venous blood samples were collected from eight healthy beagle dogs. Samples were placed in 5-mL tubes and imaged within 3 hours of collection at 1 day intervals from day 1 to day 30. The following sequences were used: T1-weighted (T1W), T2-weighted (T2W), fluid-attenuated inversion recovery (FLAIR), short tau inversion recovery (STIR), and T2-star gradient-echo... -
Production of α 1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology
Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 11 89-96 (8 pages)
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation.... -
Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs
Cho-Hee Jee, Na-Young Eom, Hyo-Mi Jang, Hae-Won Jung, Eul-Soo Choi, Jin-Hee Won, Il-Hwa Hong, Byeong-Teck Kang, Dong Wook Jeong, 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 10 79-87 (9 pages)
This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days 0,... -
A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Feng Wen, Ji-Hong Ma, Hai Yu, Fu-Ru Yang, Meng Huang, Yan-Jun Zhou, Ze-Jun Li, Xiu-Hui Wang, Guo-Xin Li, Yi-Feng Jiang, Wu Tong, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 9 71-78 (8 pages)
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by...


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