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Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 14 541-545 (5 pages)
In this study, we examine prevalences of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma, and Toxoplasma gondii) in feral cats from urban and rural habitats. Infection status of the three pathogens in blood samples (n = 117) was determined through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, Toxoplasma gondii, and Bartonella henselae was 47.9%, 50%, and 35.7%, respectively. Comparing the two habitats, only... -
A serine/threonine phosphatase 1 of Streptococcus suis type 2 is an important virulence factor
Lihua Fang, Jingjing Zhou, Pengcheng Fan, Yunkai Yang, Hongxia Shen, Weihuan Fang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 3 439-447 (9 pages)
in a single reaction step. This study investigated the role of stp1 in the pathogenesis of SS2. An isogenic stp1 mutant (Δstp1) was constructed from SS2 strain ZJ081101. The Δstp1 mutant exhibited a significant increase in adhesion to HEp-2 and bEnd.3 cells as well as increased survival in RAW264.7 cells, as compared to the parent strain. Increased survival in macrophage cells might be related to resistance to reactive oxygen species since the Δstp1 mutant was more resistant than its... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test... -
Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-hyun Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 4 283-289 (7 pages)
Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis,... -
Phenotypic and genotypic antimicrobial resistance and virulence genes of Salmonella enterica isolated from pet dogs and cats
Songsak Srisanga, Sunpetch Angkititrakul, Patcharee Sringam, Phuong T. Le Ho, An T. T. Vo, Rungtip Chuanchuen 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 3 273-281 (9 pages)
Salmonella enterica isolates (n = 122), including 32 serotypes from 113 dogs and 9 cats, were obtained from household dogs (n = 250) and cats (n = 50) during 2012?2015. The isolates were characterized by serotyping, antimicrobial resistance phenotyping and genotyping, and virulence gene screening. Serovars Weltevreden (15.6%) and Typhimurium (13.9%) were the most common. The majority (43%) of the isolates were multidrug resistant. The dog isolates (12.3%) harbored class 1 integrons, of which the... -
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex... -
Antigenic characterization of classical swine fever virus YC11WB isolates from wild boar
Seong-In Lim, Yong Kwan Kim, Ji-Ae Lim, Song-Hee Han, Hee-Suk Hyun, Ki-Sun Kim, Bang-Hun Hyun, Jae-Jo Kim, In-Soo Cho, Jae-Young 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 10 201-207 (7 pages)
Classical swine fever (CSF), a highly contagious disease that affects domestic pigs and wild boar, has serious economic implications. The present study examined the virulence and transmission of CSF virus strain YC11WB (isolated from a wild boar in 2011) in breeding wild boar. Virulence of strain YC11WB in domestic pigs was also examined. Based on the severe clinical signs and high mortality observed among breeding wild boar, the pathogenicity of strain YC11WB resembled that of typical acute... -
Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer
Farruk M. Lutful Kabir, Patricia DeInnocentes, Payal Agarwal, Christopher P. Mill, David J. Riese 2nd, R. Curtis Bird 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 4 149-158 (10 pages)
Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse... -
Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation
Sara Perteghella, Alessandro Gaviraghi, Silvia Cenadelli, Valeria Bornaghi, Andrea Galli, Barbara Crivelli, Barbara Vigani, Dani 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 11 81-88 (8 pages)
The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability... -
Inhibition by miR-410 facilitates direct retinal pigment epithelium differentiation of umbilical cord blood-derived mesenchymal stem cells
Soon Won Choi, Jae-Jun Kim, Min-Soo Seo, Sang-Bum Park, Tae-Hoon Shin, Ji-Hee Shin, Yoojin Seo, Hyung-Sik Kim, Kyung-Sun Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 8 59-65 (7 pages)
Retinal pigment epithelium (RPE) is a major component of the eye. This highly specialized cell type facilitates maintenance of the visual system. Because RPE loss induces an irreversible visual impairment, RPE generation techniques have recently been investigated as a potential therapeutic approach to RPE degeneration. A microRNA-based technique is a new strategy for producing RPE cells from adult stem cell sources. Previously, we identified that antisense microRNA-410 (anti-miR-410) induces RPE...


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