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Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity
Tae Myoung Kim, Sang-Kyung Shin, Tae-Wang Kim, So-Young Youm, Dae Joong Kim, Byeongwoo Ahn* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 2 7-13 (7 pages)
Control of inflammation is widely accepted as an important strategy for cancer chemoprevention. Anti-inflammatory effects of bark extracts of elm tree (BEE) have been amply reported. Therefore, BEE may be a good candidate cancer chemopreventive agent. Considering the high incidence of hepatic cancer and limited therapeutic approaches for treating this disease, it is important to develop liver cancer-specific chemopreventive agents. To evaluate the chemopreventive potential of BEE, we... -
Post-mortem re-cloning of a transgenic red fluorescent protein dog
So Gun Hong, Ok Jae Koo, Hyun Ju Oh, Jung Eun Park, Minjung Kim, Geon-A Kim, Eun Jung Park, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 15 405-407 (3 pages)
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although... -
Molecular characterization of a 13-amino acid deletion in VP1 (1D) protein and novel amino acid substitutions in 3D polymerase protein of foot and mouth disease virus subtype A/Iran87
Majid Esmaelizad*, Saber Jelokhani-Niaraki, Khadije Hashemnejad, Morteza Kamalzadeh, Mohsen Lotfi 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 9 363-371 (9 pages)
The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3D) coding regions of the foot and mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (∼150 passages), was determined and aligned with previously published FMDV serotype A sequences. Overall analysis of the amino acid substitutions revealed that the partial 3D coding region contained four amino acid alterations. Amino acid sequence comparison of the VP1 coding region of the field isolates revealed... -
Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs
Gulay Ciftci*, Gul Fatma Yarim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 4 325-331 (7 pages)
In this study, we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed... -
Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice
Arash Minai-Tehrani, Young-Chan Park, Soon-Kyung Hwang, Jung-Taek Kwon, Seung-Hee Chang, Sung-Jin Park, Kyeong-Nam Yu, Ji-Eun Ki 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 2 309-317 (9 pages)
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using... -
Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs
Md. Rafiqul Alam*, Joong Ryong Ji, Min Su Kim, Nam Soo Kim 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 11 273-280 (8 pages)
The levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in synovial fluid (SF) and serum in cases of canine osteoarthritis (OA) were measured. OA was induced by a surgically-created medial patellar luxation in the left stifle of 24 dogs. SF and blood samples were collected at 1.5- and 3-month intervals, respectively. Every 3 months, one dog was euthanatized to collect tissue samples from both... -
Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa
Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 10 267-272 (6 pages)
of sperm presenting atypical acrosome reactions (from 28.38 to 4.84%) and increased the percentage of sperm exhibiting damage in the acrosome (from 6.14 to 64.26%). The percentage of sperm with typical acrosome reactions was not significantly different before (3.70%) and after (0.67%) centrifugation. TEM distinguished four different types of acrosomal status and enabled ultrastructural characterization of acrosomal injuries. The percentage of sperm exhibiting normal acrosomes decreased and... -
Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles
Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)
mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10, 10, 10 M) inhibited (p < 0.05) FSH-induced survival, growth, antrum formation, estradiol production, and oocyte apoptosis in a dose-dependent manner. However, a lower dose of SNP (10 M) significantly stimulated (p < 0.05) the survival, growth, antrum formation, follicular oocyte maturation, and stimulated progesterone secretion compared to the control. A combination... -
Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows
Felix Diaz González*, Rodrigo Muiño, Víctor Pereira, Rómulo Campos, José Luis Benedito 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 8 251-255 (5 pages)
14 mid lactation cows from four different herds with similar husbandry characteristics in Galicia, Spain. Blood samples were obtained to measure beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase. Cows in early lactation had higher levels of BHB and NEFA than mid lactation cows. High lipomobilization (NEFA > 400 mmol/L) was detected in 67% and 7% of early lactation and mid lactation... -
Multidetector computed tomographic angiography evaluation of micropig major systemic vessels for xenotransplantation
Jung Min Ryu, Woong Yoon, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Ho Jae Han* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 2 209-214 (6 pages)
vessels in micropigs and compared our results to published human data. The main vasculature of the animals was similar to that of humans, except for the iliac arterial system. However, diameters of the major systemic vessels were significantly different between micropigs and humans. Specifically, the diameter of the aortic arch, abdominal aorta, external iliac artery, and femoral artery, were measured as 1.50 ± 0.07 cm, 0.85 ± 0.06 cm, 0.52 ± 0.05 cm, and 0.48 ± 0.05 cm,...


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