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171.

Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 저
...
- 3,000원
The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with novobiocin (MKTTn), on real-time PCR was confirmed. The inhibition of PCR was statistically significant (p ＜ 0.05) in RV and MKTTn, as compared with buffered peptone water (BPW) or phosphate-buffered...
172.

The anti-thrombotic activity of surfactins

Jong hwan Lim, Byung kwon Park, Myoung seok Kim, Mi Hyun Hwang, Man Hee Rhee, Seung Chun Park, Hyo in Yun 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 15 353-355 (3 pages)

Jong hwan Lim, Byung kwon Park, Myoung seok Kim, Mi Hyun Hwang, Man Hee Rhee, Seung Chun Park, Hyo in Yun 저
...
- 1,500원
Platelet aggregation was inhibited and the density of platelet-rich plasma (PRP) clots was decreased by the preincubation of PRP with surfactins, an acidic lipopeptide of Bacillus subtilis complex BC1212 isolated from soybean paste, in dose-dependent manner. Our findings suggest that surfactins are able to prevent a platelet aggregation leading to an inhibition of additional fibrin clot formation, and to enhance fibrinolysis with facilitated diffusion of fibrinolytic agents.
173.

Characterization of Brachyspira hyodysenteriae isolates from Korea

Tae Jung Kim, Suk Chan Jung, Jae Il Lee 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 12 335-339 (5 pages)

Tae Jung Kim, Suk Chan Jung, Jae Il Lee 저
...
- 1,500원
This study was done to characterize diversity in 10 Brachyspira hyodysenteriae isolates in Korea. The isolates were compared with 14 well-characterized non-Korean strains of various Brachyspira species. All Korean isolates showed strong beta haemolysis and had blunt cell ends with 7~14 periplasmic flagella. They produced indole, and did not ferment fructose. They were alpha-glucosidase positive and alpha-galatosidase negative using the APIZYM kit. Using polyclonal antisera raised in rabbits...
174.

Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 저
...
- 3,000원
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs...
175.

Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD

Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)

Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 저
...
- 4,200원
Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing E. coli (STEC) and E. coli O157:H7 in foods and animals in Korea made an assessment of the risks difficult, and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157:H7 Rapid kit. For the...
176.

Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli)

Sung-hyun Kang, Gee-wook Shin, Yong-seung Shin, Palaksha K. J., Young-rim Kim, Hyang-hee Yang, Eun-young Lee, Eung-goo Lee, Nam- 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 15 387-390 (4 pages)

Sung-hyun Kang, Gee-wook Shin, Yong-seung Shin, Palaksha K. J., Young-rim Kim, Hyang-hee Yang, Eun-young Lee, Eung-goo Lee, Nam- 저
...
- 1,500원
Black rockfish (Sebastes schlegeli) is an important mariculture species in Korea. The production of this fish is drastically declined due to bacterial diseases, particularly streptococcosis caused by Lactococcus garvieae. The bacterial surface characteristics of SJ7 and TY6 were found to have capsule but not NB13 and YS18. The experiential evaluation of L. garvieae pathogenicity, the capsular isolates showed high cumulative mortality i.e. SJ7 (100%) and TY6 (60%) compared to non-capsular...
177.

Requirement of Metabolic Activation for Estrogenic Activity of Pueraria mirifica

Y.S. Lee, J.S. Park, S.D. Cho, J.K. Son, W. Cherdshewasart and K.S. Kang 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 4 273-277 (5 pages)

Y.S. Lee, J.S. Park, S.D. Cho, J.K. Son, W. Cherdshewasart and K.S. Kang 저
...
- 1,500원
A wide range of chemicals derived from plant and human-made xenobiotics are reported to have hormonal activities. The present study was performed to examine the estrogenic effect of Kwao Keur, Pueraria mirifica (PM), that has been used as a rejuvenating folk medicine in Thailand, using recombinant yeast, MCF-7 cell proliferation and HepG2 cell transient transfection assay. In recombinant yeast assay, 0.025, 0.25, 2.5, 25, 2.5 x 10(2), 2.5 x 10(3), 2.5 x 10(4) ng/ml concentrations of PM did not...
178.

Prevalence and Clinical Characterization of Gastric Helicobacter Species Infection of Dogs and Cats in Korea

Cheol-Yong Hwang, Hong-Ryul Hanand Hwa-Young Youn 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 10 123-134 (12 pages)

Cheol-Yong Hwang, Hong-Ryul Hanand Hwa-Young Youn 저
...
- 4,100원
This study was carried out to evaluate the prevalence and clinical characterizations of gastric Helicobacter spp. infection of dogs and cats in Korea. The prevalence of Helicobacter spp. infection of dogs and cats determined by urease test was 78.4% and 64%, respectively, although Helicobacter genus-specific PCR assay showed that it was 82.3% and 84%. Urease mapping results based on urease test showed that total positive rate of tested tissues from clinically abnormal dogs was significantly...
179.

Efficacy of Purified Vi Polysaccharide Typhoid Vaccine

Jong-Hwan Park, Jung-Joo Hong, Eun-Sil Choi, Jin-Won Leeand Jae-Hak Park 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 2 67-70 (4 pages)

Jong-Hwan Park, Jung-Joo Hong, Eun-Sil Choi, Jin-Won Leeand Jae-Hak Park 저
...
- 1,500원
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microg / 0.5 ml / mouse. The ELISA titer (0.50970.0606) was 4 times in the group treated with high dose (0.25 microg/0.5ml) as in control (0.11130.0110). Six...
180.

Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae

Jong Man Kim, Han Sang Yoo, In Soo Choi, Won Hur, Na Ri Shin 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 8 47-57 (11 pages)

Jong Man Kim, Han Sang Yoo, In Soo Choi, Won Hur, Na Ri Shin 저
...
- 4,000원
Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems...

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