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Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma
Seungmo Park, Jihae Lim, Jong Rhan Kim, Seongbeom Cho 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 1 419-429 (11 pages)
by using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell-sorter analysis showed that resveratrol induced G1 cell cycle arrest without increasing the sub-G1 phase cell population. Therefore, we evaluated its effect on regulation of cyclin D1, which is critically involved in G1/S transition. Resveratrol lowered cyclin D1 transcription. Western blot analysis of the effects of resveratrol on... -
Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
Hyeon-Joong Kim, Joon Young Kim, Byung-Hwan Lee, Sun-Hye Choi, Hyewon Rhim, Hyoung-Chun Kim, Seoung-Yob Ahn, Soon-Wuk Jeong, Min 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 16 387-397 (11 pages)
a rabbit model of corneal damage, by undertaking histochemical analysis. Treatment of gintonin to HCE cells induced transient increases of [Ca]i in concentration-dependent and reversible manners. Gintonin-mediated mobilization of [Ca]i was attenuated by LPA1/3 receptor antagonist Ki16425, phospholipase C inhibitor U73122, inositol 1,4,5-triphosphate receptor antagonist 2-APB, and intracellular Ca chelator BAPTA-AM. Gintonin facilitated in vitro wound healing in a concentration-dependent manner.... -
Effect of phosphorus deficiency on erythrocytic morphology and function in cows
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 10 333-340 (8 pages)
The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly... -
Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro
Junyuan Han, Shuxia Zhang, Yaqun Zhang, Mengmeng Chen, Yingjun Lv 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 8 183-191 (9 pages)
Porcine alveolar macrophages (PAMs) represent the first line of defense in the porcine lung after infection with porcine circovirus type 2 (PCV2) via the respiratory tract. However, PCV2 infection impairs the microbicidal capability of PAMs and alters cytokine production and/or secretion. At present, the reason for the imbalance of cytokines has not been fully elucidated, and the regulatory mechanisms involved are unclear. In this study, we investigated the expression levels and regulation of... -
Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin
Rafael Herrera Alvarez, Fabio Luis Nogueira Natal, Maria Teresa Carvalho Pinto Ribela, Beatriz Elane de Almeida, Jo?o Ezequiel d 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 4 459-465 (7 pages)
this qualitative and quantitative physical?chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR = 27.9 min), which were related to the in vivo bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate... -
Hematoporphyrin monomethyl ether combined with He?Ne laser irradiation-induced apoptosis in canine breast cancer cells through the mitochondrial pathway
Huatao Li, Jinjin Tong, Jun Bao, Damu Tang, Wenru Tian, Yun Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 13 235-242 (8 pages)
was further confirmed by Real-time-polymerase chain reaction and Western blotting assays. Our results showed that HMME-PDT induced significant changes in cell morphology, such as formation of cytoplasmic vacuoles and the gradual rounding of cells coupled with decreased size and detachment. DNA fragmentation and cell death was shown to occur in a time-dependent manner. Furthermore, HMME-PDT increased the activities of caspase-9 and caspase-3, and released cytochrome c from mitochondria into the... -
Effects of induced endometritis on uterine blood flow in cows as evaluated by transrectal Doppler sonography
Letizia Debertolis, Gaetano Mari, Barbara Merlo, Sabine Merbach, Heinz-Adolf Schoon, Eleonora Iacono, Heinrich Bollwein 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 8 189-197 (9 pages)
This study was conducted to evaluate the effects of induced endometritis on uterine blood flow in cows. Transrectal Doppler sonography was performed on uterine arteries of six cyclic cows before and for 4 days after inducing acute endometritis by intrauterine infusion of 720 mg of policresulen, and for 4 days of the following estrous cycle. Time-averaged maximum velocity (TAMV) increased (p < 0.001) and pulsatility index (PI) decreased (p < 0.0001) within 1 h of policresulen... -
Assessment of the safety and efficacy of low pathogenic avian influenza (H9N2) virus in inactivated oil emulsion vaccine in laying hens
Jeong-Hwa Shin, Jong Seo Mo, Jong-Nyeo Kim, In-pil Mo, Bong-Do Ha 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 4 27-34 (8 pages)
and conducted safety and efficacy tests in commercial layer farms while focusing on analysis of factors that cause losses to farms, including egg production rate, egg abnormality, and feed efficiency. The egg production rate of the control group declined dramatically 5 days after the challenge. There were no changes in feed consumption of all three groups before the challenge, but rates of the control declined afterward. Clinical signs in the vaccinated groups were similar, and a slight decline... -
Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin
Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)
direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the developed ELISA in the food matrix, known amounts of ENR... -
Cadmium induces apoptosis in primary rat osteoblasts through caspase and mitogen-activated protein kinase pathways
Hongyan Zhao, Wei Liu, Yi Wang, Nannan Dai, Jianhong Gu, Yan Yuan, Xuezhong Liu, Jianchun Bian, Zong-Ping Liu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 7 297-306 (10 pages)
Exposure to cadmium (Cd) induces apoptosis in osteoblasts (OBs); however, little information is available regarding the specific mechanisms of Cd-induced primary rat OB apoptosis. In this study, Cd reduced cell viability, damaged cell membranes and induced apoptosis in OBs. We observed decreased mitochondrial transmembrane potentials, ultrastructure collapse, enhanced caspase-3 activity, and increased concentrations of cleaved PARP, cleaved caspase-9 and cleaved caspase-3 following Cd treatment....


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