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181.

Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 저
...
- 3,000원
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal...
182.

Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos

Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)

Kilyoung Song, Eunsong Lee 저
...
- 3,000원
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was...
183.

Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen

Hye Jin Kim, Hyun Ju Oh, Goo Jang, Min Kyu Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 11 75-80 (6 pages)

Hye Jin Kim, Hyun Ju Oh, Goo Jang, Min Kyu Kim 저
...
- 3,000원
The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate...
184.

Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island

Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 8 51-55 (5 pages)

Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 저
...
- 1,500원
Swine hepatitis E virus (HEV) is an emerging zoonotic pathogen due to its close genomic similarity to human HEV. The prevalence of swine HEV in the hepatic lesion of pigs from the Jeju Island was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). In total, 40 pigs with hepatitis lesions were selected from 19 different farms, based on examination by microscopy. RTPCR findings revealed swine HEV in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. Several...
185.

Comparative analysis of heart functions in micropigs and conventional pigs using echocardiography and radiography

Min Young Lee, Sang Hun Lee, Seung Gon Lee, Soo Hyun Park, Chai Yong Lee, Kye Hun Kim, Sun Ho Hwang, Sang Yub Lim, Young Keun Ah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 2 7-14 (8 pages)

Min Young Lee, Sang Hun Lee, Seung Gon Lee, Soo Hyun Park, Chai Yong Lee, Kye Hun Kim, Sun Ho Hwang, Sang Yub Lim, Young Keun Ah 저
...
- 3,000원
The production of miniature animals has been suggested for use in organ transplantation. At present, many of the studies about application of animal organs to human have been focused on pigs because of the number of advantages involved and due to their similarities with human. However, a physiological analysis of the organs to be transplanted has not yet been carried out. Therefore, this study analyzed whether or not there were physiological and morphological differences in the hearts of...
186.

Attenuation of ischemia-reperfusion injury by ascorbic acid in the canine renal transplantation

Jae-il Lee, Hwa-Young Son, Myung-cheol Kim 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 12 375-379 (5 pages)

Jae-il Lee, Hwa-Young Son, Myung-cheol Kim 저
...
- 1,500원
This study examined the effects of ascorbic acid on the attenuation of an ischemia-reperfusion (I/R) injury after a canine renal transplantation. Eight beagle dogs were subjected to a renal auto-transplantation followed by the administration of ascorbic acid (treatment group) and the same amount of vehicle (physiological saline, control group). Blood samples were collected from these dogs to perform the kidney function tests and the invasive blood pressure was measured in the renal artery at...
187.

Guided bone regeneration with beta-tricalcium phosphate and poly L- lactide-co-glycolide-co-epsilon-caprolactone membrane in partial defects of canine humerus

Taehoon Oh , Md.Mizanur Rahman , Ji-Hey Lim , Mi-Sun Park , Dae-Yong Kim , Jung-hee Yoon , Wan Hee Kim , Masanori Kikuchi , Junz 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 13 73-77 (5 pages)

Taehoon Oh , Md.Mizanur Rahman , Ji-Hey Lim , Mi-Sun Park , Dae-Yong Kim , Jung-hee Yoon , Wan Hee Kim , Masanori Kikuchi , Junz 저
...
- 1,500원
This study was performed to evaluate the effect of beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone (TCP/PLGC)membrane in the repair of partial bone defects in canine proximal humerus. Three adult mixed-breed dogs were used during the experimental period. The length of the defect was quarter of the full length of humerus, and width of the defect was quarter of middle diameter of the lateral aspect of humerus. The humeri of each dog were divided into treatment...
188.

Successful surgical correction of anal atresia in a transgenic cloned piglet

Gab Sang Lee, Hye Soo Kim, So Hyun Lee, Dae Yong Kim, Kang Moon Seo, Sang Hwan Hyun, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hw 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 12 243-245 (3 pages)

Gab Sang Lee, Hye Soo Kim, So Hyun Lee, Dae Yong Kim, Kang Moon Seo, Sang Hwan Hyun, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hw 저
...
- 1,500원
Inbred strains of pig become indispensable for a wide range of biological studies. In biomedical science, it is generally accepted that somatic cell nuclear transfer(SCNT)technology with inbreed strain of pig is essential for xenotransplantation. In this study, we observed the anal atresia in a cloned pig which was derived from fetal fibroblast of inbreed miniature pig. A presumptive anal site of the cloned pig was excised and the rectum was sutured to apposed skin for treatment. This cloned...
189.

Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes

Yong-Seung Shin, Eung-Goo Lee, Tae-Sung Jung 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 11 157-160 (4 pages)

Yong-Seung Shin, Eung-Goo Lee, Tae-Sung Jung 저
...
- 1,500원
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were exhibited on immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. A 46 kDa band was probed as a common antigenic band except IgA; 69 kDa band was bovine IgM and IgE; 33, 37, 55, and 79 kDa bands were bovine IgM and IgG; 72 kDa band was found IgM and IgA profiles. Based on the analysis,...
190.

Human embryonic stem cells and therapeutic cloning

Woo Suk Hwang, Byeong Chun Lee, Chang Kyu Lee, Sung Keun Kang 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 1 87-96 (10 pages)

Woo Suk Hwang, Byeong Chun Lee, Chang Kyu Lee, Sung Keun Kang 저
...
- 3,000원
The remarkable potential of embryonic stem (ES) cells is their ability to develop into many different cell types. ES cells make it possible to treat patients by transplanting specialized healthy cells derived from them to repair damaged and diseased cells or tissues, known as “stem cell therapy”. However, the issue of immunocompatibility is one of considerable significance in ES cell transplantation. One approach to overcome transplant rejection of human ES (hES) cells is to derive hES cells...

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