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181.

Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes

Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 2 285-292 (8 pages)

Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 저
...
- 3,000원
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3...
182.

Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 7 219-223 (5 pages)

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 저
...
- 1,500원
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and...
183.

Protective efficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recent Korean epizootic strain

Woo-Jin Jeon, Eun-Kyoung Lee, Young-Jeong Lee, Ok-Mi Jeong, Yong-Joo Kim, Jun-Hun Kwon, Kang-Seuk Choi 대한수의학회 Journal of Veterinary Science

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 10 295-294 (0 pages)

Woo-Jin Jeon, Eun-Kyoung Lee, Young-Jeong Lee, Ok-Mi Jeong, Yong-Joo Kim, Jun-Hun Kwon, Kang-Seuk Choi 저
...
- 무료
Despite the intensive vaccination policy that has been put in place to control Newcastle disease virus (NDV), the recent emergence of NDV genotype VII strains in Korea has led to significant economic losses in the poultry industry. We assessed the ability of inactivated, oil-emulsion vaccines derived from La Sota or Ulster 2C NDV strains to protect chickens from challenge with Kr-005/00, which is a recently isolated Korean epizootic genotype VII strain. Six-week-old SPF chickens were vaccinated...
184.

Tactics of Mycobacterium avium subsp. paratuberculosis for intracellular survival in mononuclear phagocytes

Seng-Ryong Woo, Charles J. Czuprynski* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 1 1-8 (8 pages)

Seng-Ryong Woo, Charles J. Czuprynski* 저
...
- 3,000원
Johne's disease is a condition that refers to chronic granulomatous enteritis in ruminants. It is believed that survival and replication of Mycobacterium (M.) paratuberculosis in mononuclear phagocytes plays an important role in the pathogenesis of Johne's disease. However, it is not clear how M. paratuberculosis survives for long time periods in mononuclear phagocytes, nor is it clear which factors trigger multiplication of these bacilli and result in the development of Johne's disease....
185.

Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats

Heechul Kim, Changjong Moon, Jeongtae Kim, Meejung Ahn, Jin Won Hyun, Jae Woo Park, Sung-Ho Kim, Seungjoon Kim, Taekyun Shin* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 2 323-328 (6 pages)

Heechul Kim, Changjong Moon, Jeongtae Kim, Meejung Ahn, Jin Won Hyun, Jae Woo Park, Sung-Ho Kim, Seungjoon Kim, Taekyun Shin* 저
...
- 3,000원
Phosphorylation of caveolin-1 occurs during cell activation by various stimuli. In this study, the involvement of caveolin-1 in an irradiation injured spinal cord was examined by analyzing the phosphorylation of caveolin-1 in the spinal cord of rats after irradiation with a single dose of 15 Gray from a Co ■-ray source at 24 h post-irradiation (PI). A Western blot analysis showed that the phosphorylated form of caveolin-1 (p-caveolin-1) was expressed constitutively in the normal spinal cords...
186.

Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos

Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)

Kilyoung Song, Eunsong Lee 저
...
- 3,000원
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was...
187.

Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 저
...
- 3,000원
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to...
188.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 저
...
- 3,000원
maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
189.

Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR

Khalid Munir 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 13 167-176 (10 pages)

Khalid Munir 저
...
- 3,000원
We have previously described the development of a onetube SYBR Green real-time RT-PCR assay for the detection and quantitation of infectious salmon anemia virus (ISAV) in various biological samples. The twofold aim of the present study was to verify that the optimized SYBR Green real-time RT-PCR conditions could detect ISAV isolates of different geographic origins, and to analyze the growth patterns of the selected ISAV isolates in the Chinook head salmon embryo (CHSE) -214 cells by this assay...
190.

Development of immunoassays for the detection of kanamycin in veterinary fields

Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 3 111-117 (7 pages)

Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 저
...
- 3,000원
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of...

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