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181.

Purification and Characterization of a Major Extracellular Chitinase from a Biocontrol Bacterium, Paenibacillus elgii HOA73

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 10 318-328 (11 pages)

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- 4,000원
Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type...
182.

Characterization of the rcsA Gene from Pantoea sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on Pleurotus eryngii

한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 6 276-287 (12 pages)

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- 4,100원
RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins....
183.

Potential Reasons for Prevalence of Fusarium Wilt in Oriental Melon in Korea

한국식물병리학회 The Plant Pathology Journal 15 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 4 249-263 (15 pages)

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- 4,400원
This study aims to examine the potential reasons for the current prevalence of the fusarium wilt in the oriental melon. Twenty-seven Fusarium isolates obtained from oriental melon greenhouses in 2010–2011 were identified morphologically and by analysis of elongation factor-1 alpha gene (EF-1α) and internal transcribed spacer (ITS) rDNA sequences as 6 Fusarium species (8 isolates of F. oxysporum, 8 F. commune, 5 F. proliferatum, 3 F. equiseti, 2 F. delphinoides, and 1 F....
184.

Enhancement of Seed Dehiscence by Seed Treatment with Talaromyces flavus GG01 and GG04 in Ginseng (Panax ginseng)

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 1호 1 1-8 (8 pages)

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- 3,000원
Seed dehiscence of ginseng (Panax ginseng C. A. Mayer) is affected by moisture, temperature, storage conditions and microbes. Several microbes were isolated from completely dehisced seed coat of ginseng cultivars, Chunpoong and Younpoong at Gumsan, Korea. We investigated the potential of five Talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. The isolates showed antagonistic activities against fungal plant pathogens, such as...
185.

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 4 519-527 (9 pages)

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- 3,000원
Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession ‘CBT-Ac77’ and cultivar ‘Arka Kalyan’ were observed to be highly...
186.

Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 9 452-459 (8 pages)

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- 3,000원
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate...
187.

Occurrence of Toxigenic Fusarium vorosii among Small Grain Cereals in Korea

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 4 407-413 (7 pages)

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- 3,000원
Fusarium graminearum species complex (FGSC) causes Fusarium head blight in small grain cereals. To date, four species (F. graminearum, F. asiaticum, F. boothii, and F. meridionale ) belonging to FGSC frequently occur in Korean cereals. In addition, we first reported the occurrence of additional species (F. vorosii ) within FGSC, which was isolated from barley, corn, and rice in Korea. Phylogenetic analysis of the Fusarium isolates of this group using combined multi-gene sequences confirmed...
188.

The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 4호 5 321-328 (8 pages)

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- 3,000원
We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of...
189.

Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 4 201-208 (8 pages)

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- 3,000원
using previously described TYLCV-specific primer pair TYLCV-Sar/TYLCV-Isr or universal primer pair Begomo-F/Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCV-specific primers, the PCR reaction using...
190.

Characterization of Nivalenol-Producing Fusarium culmorum Isolates Obtained from the Air at a Rice Paddy Field in Korea

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 2 182-189 (8 pages)

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- 3,000원
derived from five genes encoding translation elongation factor, histone H3, phosphate permease, a reductase, and an ammonia ligase and by morphological comparison with reference strains. Both diagnostic PCR and chemical analysis confirmed that these F. culmorum isolates had the capacity to produce nivalenol, the trichothecene mycotoxin, in rice substrate. In addition, both isolates were pathogenic on wheat heads and corn stalks. This is the first report on the occurrence of F. culmorum in Korea.

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