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Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Woo Bin Park, Young Bin Im, Soojin Shim, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 16 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 8 216-231 (16 pages)
Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonoses. Pathogenesis of the B. abortus infection is complicated, and several researchers have attempted to elucidate the infection mechanism of B. abortus. While several proteins have been revealed as pathogenic factors by previous researchers, the underlying mechanism of B. abortus infection is unresolved. In this study, we identified proteins showing different expression levels in B. abortus... -
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than... -
Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus
Eun-Ju Kim, Kwang-Myun Cheong, Ha-Kyung Joung, Bo-Hye Kim, Jae-Young Song, In-Soo Cho, Kyoung-Ki Lee, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 6 479-487 (9 pages)
Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the... -
Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
Hyojin Lee, Eun-Ju Kim, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, In-Soo Cho, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 6 307-314 (8 pages)
Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are... -
Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice
Jine Yi*, Wei Xia, Jianping Wu, Liyun Yuan, Jing Wu, Di Tu, Jun Fang, Zhuliang Tan* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 16 141-148 (8 pages)
Betulinic acid (BA), a pentacyclic lupane-type triterpene, has a wide range of bioactivities. The main objective of this work was to evaluate the hepatoprotective activity of BA and the potential mechanism underlying the ability of this compound to prevent liver damage induced by alcohol in vivo. Mice were given oral doses of BA (0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and induced liver injury by feeding 50% alcohol orally at the dosage of 10 ml/kg after 1 h last administration of BA. BA... -
Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects
Byung-Jae Kang, Hak-Hyun Ryu, Sung Su Park, Yoshihisa Koyama, Masanori Kikuchi, Heung-Myong Woo, Wan Hee Kim, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 12 299-310 (12 pages)
Alternative sources of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field of bone tissue engineering. The purpose of this study was to compare the osteogenic potential of canine MSCs derived from adipose tissue (AT), BM, umbilical cord blood (UCB), and Wharton's jelly (WJ) using in vitro culture techniques and in vivo orthotopic implantation assays. After canine MSCs were isolated from various tissues, the proliferation and osteogenic... -
Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles
Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)
Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200∼250 mm) were isolated by micro-dissection and cultured in 0 (control), 10, 10, 10, and 10 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10 M SNP + 1 mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10,... -
Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD
Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)
Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing E. coli (STEC) and E. coli O157:H7 in foods and animals in Korea made an assessment of the risks difficult, and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157:H7 Rapid kit. For the... -
Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System
Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)
The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been known to be a 24.5-26 kDa N-glycosylated envelope protein. The GP5 is involved in inducing neutralizing antibodies. For this reason, the GP5 is primary candidate for the PRRSV subunit vaccine. To produce the native form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki...


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