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11.

Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi

Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 7 162-172 (11 pages)

Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 저
...
- 4,000원
can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross- family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly tech- niques to carry out SMV full-length genome amplifi- cation from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes...
12.

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 9 468-475 (8 pages)

Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 저
...
- 3,000원
rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids....
13.

Characterization of the Rosellinia necatrix Transcriptome and Genes Related to Pathogenesis by Single-Molecule mRNA Sequencing

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 2 362-369 (8 pages)

저
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- 3,000원
of R. necatrix (KACC 40445 strain) using PacBio single-molecule sequencing technology. After annotation of the unigene sets, we selected 14 cell cycle-related genes, which are likely either positively or negatively involved in hyphal growth by cell cycle control. The expression of the selected genes was further compared between two strains that displayed different growth rates on nutritional media. Furthermore, we predicted pathogen-related effector genes and cell wall-degrading enzymes from the...
14.

Deep Sequencing Analysis of Apple Infecting Viruses in Korea

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 8 441-451 (11 pages)

저
...
- 4,000원
Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes....
15.

Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)

저
...
- 3,000원
a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSVRNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/ RE, sequence and phylogenetic analyses, indicating...
16.

Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 12 83-89 (7 pages)

저
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- 3,000원
Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0%...
17.

식물 RNA 바이러스의 진화와 병저항성 극복 기작

김명휘, 권선정, 서장균 한국식물병리학회 식물병연구 12 Pages

한국식물병리학회 식물병연구 2021, 27권 4호 2 137-148 (12 pages)

김명휘, 권선정, 서장균 저
...
- 4,100원
18.

Transient Expression of Homologous Hairpin RNA Interferes with Broad bean wilt virus 2 Infection in Nicotiana benthamiana

한국식물병리학회 식물병연구 9 Pages

한국식물병리학회 식물병연구 2012, 18권 4호 2 268-276 (9 pages)

저
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- 3,000원
19.

Targeted RNA Recombination of the Membrane Nucleocapsid Protein Genes between Mouse Hepatitis Virus Bovine Coronavirus

Gabriella Koljesar, Dong Wan Yoo 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 1 149-157 (9 pages)

Gabriella Koljesar, Dong Wan Yoo 저
...
- 3,000원
4 followed by transfection with synthetic RNA transcribed from the DI-like cDNA construct, recombinant mouse hepatitis viruses as the large plaque forming phenotype were isolated by plaque assays at the non-permissive temperature of 391 degrees C. By RT-PCR and sequencing, those large plaque phenotypes were confirmed to have contained the thermostable phenotype marker derived from the transfected RNA, demonstrating that recombination occurred between the Albany 4 genomic RNA and the in vitro RNA...
20.

Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus

한국식물병리학회 The Plant Pathology Journal 3 Pages

한국식물병리학회 The Plant Pathology Journal 2004, 20권 4호 16 313-315 (3 pages)

저
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- 1,500원
isolate of Rice stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a...

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